TY - JOUR
T1 - Mechanical and Geometric Characterization of a Novel 2-Ply Vacuum-Pressed Biological Scaffold Patch Design for Posterior Mitral Valve Reconstruction
AU - Jedrzejczyk, Johannes H
AU - Andersen, Frederik T
AU - Petersen, Jacob
AU - Kaspersen, Alexander Emil
AU - Sahana, Urjosee
AU - Skov, Søren N
AU - Væsel, Jens T
AU - Hasenkam, J Michael
AU - Tjørnild, Marcell J
N1 - © 2024. The Author(s).
PY - 2024/10/28
Y1 - 2024/10/28
N2 - To assess the mechanical properties of small intestinal submucosal extracellular matrix (SIS-ECM) iterations and choose the optimal version for evaluating functional geometrics after posterior mitral valve reconstruction. Four SIS-ECM versions (2- and 4-ply vacuum-pressed and lyophilized) underwent uniaxial tensile testing. A posterior mitral valve reconstruction patch was developed based on MRI scans (n = 5). Posterior mitral valve reconstruction using 2-ply vacuum-pressed SIS-ECM was performed (n = 7), and geometrics were evaluated using a modified left heart simulator. The vacuum-pressed iterations displayed superior maximum stress values compared to lyophilized (2-ply: median [IQR], 15.8 [15.2-19.0] vs 7.9 [7.3-8.3] MPa, p < 0.001; 4-ply: median (IQR), 15.8 -[14.6-22.0] vs 7.9 [7.6-8.4] MPa). All reconstructed valves were competent with preserved total leaflet area, but individual leaflet segment areas were redistributed. Posterior mitral valve reconstruction with our 2-ply vacuum-pressed SIS-ECM patch design was feasible in vitro. Further in vivo evaluation is warranted.
AB - To assess the mechanical properties of small intestinal submucosal extracellular matrix (SIS-ECM) iterations and choose the optimal version for evaluating functional geometrics after posterior mitral valve reconstruction. Four SIS-ECM versions (2- and 4-ply vacuum-pressed and lyophilized) underwent uniaxial tensile testing. A posterior mitral valve reconstruction patch was developed based on MRI scans (n = 5). Posterior mitral valve reconstruction using 2-ply vacuum-pressed SIS-ECM was performed (n = 7), and geometrics were evaluated using a modified left heart simulator. The vacuum-pressed iterations displayed superior maximum stress values compared to lyophilized (2-ply: median [IQR], 15.8 [15.2-19.0] vs 7.9 [7.3-8.3] MPa, p < 0.001; 4-ply: median (IQR), 15.8 -[14.6-22.0] vs 7.9 [7.6-8.4] MPa). All reconstructed valves were competent with preserved total leaflet area, but individual leaflet segment areas were redistributed. Posterior mitral valve reconstruction with our 2-ply vacuum-pressed SIS-ECM patch design was feasible in vitro. Further in vivo evaluation is warranted.
KW - Lyophilized
KW - Mitral valve reconstruction
KW - Mitral valve repair
KW - Posterior mitral valve leaflet
KW - Small intestinal submucosal extracellular matrix
KW - Vacuum-pressed
UR - http://www.scopus.com/inward/record.url?scp=85207326720&partnerID=8YFLogxK
U2 - 10.1007/s12265-024-10572-0
DO - 10.1007/s12265-024-10572-0
M3 - Journal article
C2 - 39466570
SN - 1937-5387
JO - Journal of Cardiovascular Translational Research
JF - Journal of Cardiovascular Translational Research
ER -