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Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers

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Standard

Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. / Sørensen, Charlotte S; Kjaergaard, Magnus.

Intrinsically Disordered Proteins: Methods and protocols. red. / Birthe B. Kragelund; Karen Skriver. New York : Humana Press, 2020. s. 505-518 (Methods in Molecular Biology, Bind 2141).

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

Harvard

Sørensen, CS & Kjaergaard, M 2020, Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. i BB Kragelund & K Skriver (red), Intrinsically Disordered Proteins: Methods and protocols. Humana Press, New York, Methods in Molecular Biology, bind 2141, s. 505-518. https://doi.org/10.1007/978-1-0716-0524-0_25

APA

Sørensen, C. S., & Kjaergaard, M. (2020). Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. I B. B. Kragelund, & K. Skriver (red.), Intrinsically Disordered Proteins: Methods and protocols (s. 505-518). Humana Press. Methods in Molecular Biology Bind 2141 https://doi.org/10.1007/978-1-0716-0524-0_25

CBE

Sørensen CS, Kjaergaard M. 2020. Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. Kragelund BB, Skriver K, red. I Intrinsically Disordered Proteins: Methods and protocols. New York: Humana Press. s. 505-518. (Methods in Molecular Biology, Bind 2141). https://doi.org/10.1007/978-1-0716-0524-0_25

MLA

Sørensen, Charlotte S og Magnus Kjaergaard "Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers". og Kragelund, Birthe B. Skriver, Karen (red.). Intrinsically Disordered Proteins: Methods and protocols. New York: Humana Press. (Methods in Molecular Biology, Bind 2141). 2020, 505-518. https://doi.org/10.1007/978-1-0716-0524-0_25

Vancouver

Sørensen CS, Kjaergaard M. Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. I Kragelund BB, Skriver K, red., Intrinsically Disordered Proteins: Methods and protocols. New York: Humana Press. 2020. s. 505-518. (Methods in Molecular Biology, Bind 2141). https://doi.org/10.1007/978-1-0716-0524-0_25

Author

Sørensen, Charlotte S ; Kjaergaard, Magnus. / Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers. Intrinsically Disordered Proteins: Methods and protocols. red. / Birthe B. Kragelund ; Karen Skriver. New York : Humana Press, 2020. s. 505-518 (Methods in Molecular Biology, Bind 2141).

Bibtex

@inbook{0b86be768fae4d3fb8c82b6ea8fbaada,
title = "Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers",
abstract = "Intrinsically disordered linkers control avidity, auto-inhibition, catalysis, and liquid-liquid phase separation in multidomain proteins. Linkers enforce effective concentrations that directly affect the kinetics and equilibrium positions of intramolecular reactions. Mechanistic understanding of the role of linkers thus requires measurements of the effective concentrations in supramolecular complexes. Here, we describe an experimental protocol for measuring the effective concentrations enforced by a linker using a competition assay. The experiment uses a FRET biosensor that is titrated by a competitor peptide. The assay is designed for parallel analysis of several constructs in a fluorescent plate reader and has been used to study hundreds of synthetic disordered linkers.",
author = "S{\o}rensen, {Charlotte S} and Magnus Kjaergaard",
year = "2020",
doi = "10.1007/978-1-0716-0524-0_25",
language = "English",
isbn = "978-1-0716-0523-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "505--518",
editor = "Kragelund, {Birthe B.} and Karen Skriver",
booktitle = "Intrinsically Disordered Proteins",

}

RIS

TY - CHAP

T1 - Measuring Effective Concentrations Enforced by Intrinsically Disordered Linkers

AU - Sørensen, Charlotte S

AU - Kjaergaard, Magnus

PY - 2020

Y1 - 2020

N2 - Intrinsically disordered linkers control avidity, auto-inhibition, catalysis, and liquid-liquid phase separation in multidomain proteins. Linkers enforce effective concentrations that directly affect the kinetics and equilibrium positions of intramolecular reactions. Mechanistic understanding of the role of linkers thus requires measurements of the effective concentrations in supramolecular complexes. Here, we describe an experimental protocol for measuring the effective concentrations enforced by a linker using a competition assay. The experiment uses a FRET biosensor that is titrated by a competitor peptide. The assay is designed for parallel analysis of several constructs in a fluorescent plate reader and has been used to study hundreds of synthetic disordered linkers.

AB - Intrinsically disordered linkers control avidity, auto-inhibition, catalysis, and liquid-liquid phase separation in multidomain proteins. Linkers enforce effective concentrations that directly affect the kinetics and equilibrium positions of intramolecular reactions. Mechanistic understanding of the role of linkers thus requires measurements of the effective concentrations in supramolecular complexes. Here, we describe an experimental protocol for measuring the effective concentrations enforced by a linker using a competition assay. The experiment uses a FRET biosensor that is titrated by a competitor peptide. The assay is designed for parallel analysis of several constructs in a fluorescent plate reader and has been used to study hundreds of synthetic disordered linkers.

U2 - 10.1007/978-1-0716-0524-0_25

DO - 10.1007/978-1-0716-0524-0_25

M3 - Book chapter

C2 - 32696374

SN - 978-1-0716-0523-3

T3 - Methods in Molecular Biology

SP - 505

EP - 518

BT - Intrinsically Disordered Proteins

A2 - Kragelund, Birthe B.

A2 - Skriver, Karen

PB - Humana Press

CY - New York

ER -