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Maternal inheritance of chloroplasts between Brassica rapa and F 1-hybrids demonstrated by cpDNA markers specific to oilseed rape and B. rapa

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

Controlled reciprocal crosses between B. rapa and F1-hybrids (B. napus (♂) × B. rapa), giving 20 pair-crossings, were made to reveal possible irregularities in chloroplast inheritance during production of BC 1s. Despite the close relationship of chloroplasts in B. rapa and B. napus, development of PCR-based molecular markers specific to B. rapa chloroplasts and B. napus chloroplasts was successful. Offspring from each cross were investigated and among these, we found no irregular chloroplast inheritance, since their plastid genotypes in all cases were identical to that of their mother. With a certainty of 95% our data indicate that the probability that chloroplasts are being inherited paternally is less than 0.015. In oilseed rape, pollen-mediated transgene-dispersal poses a well-known risk. Our results support development of transplastomic oilseed rape as an approach to reduce transgene dispersal.

OriginalsprogEngelsk
TidsskriftMolecular Breeding
Vol/bind16
Nummer4
Sider (fra-til)271-278
Antal sider8
ISSN1380-3743
DOI
StatusUdgivet - 1 nov. 2005

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