Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells

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Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells. / Holst, Mikkel R; Nielsen, Simone S E; Nielsen, Morten S.

I: Methods in molecular biology (Clifton, N.J.), 20.08.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskning

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@article{7768ae288c79473d9dc24d9bc174fda9,
title = "Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells",
abstract = "Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.",
author = "Holst, {Mikkel R} and Nielsen, {Simone S E} and Nielsen, {Morten S}",
year = "2020",
month = aug,
day = "20",
doi = "10.1007/7651_2020_312",
language = "English",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells

AU - Holst, Mikkel R

AU - Nielsen, Simone S E

AU - Nielsen, Morten S

PY - 2020/8/20

Y1 - 2020/8/20

N2 - Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.

AB - Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.

U2 - 10.1007/7651_2020_312

DO - 10.1007/7651_2020_312

M3 - Journal article

C2 - 32813236

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -