Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells

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Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745
DOI
StatusE-pub ahead of print - 20 aug. 2020

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