Mapping of a major osteomagenic determinant of murine leukemia virus RFB-14 to non-long terminal repeat sequences

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  • Mette Østergaard, Danmark
  • Lene Pedersen
  • Jörg Schmidt, Institut für Molekulare Virologie, GSF-Forschungszentrum für Umwelt und Gesundheit , Tyskland
  • Arne Luz, Institut für Pathologie, GSF-Forschungszentrum für Umwelt und Gesundheit , Tyskland
  • Jette Lovmand, Danmark
  • Volker Erfle, Institut für Molekulare Virologie, GSF-Forschungszentrum für Umwelt und Gesundheit , Tyskland
  • Finn Skou Pedersen
Certain isolates of murine leukemia viruses (MuLVs) have, apart from a leukemogenic potential, the capability of inducing diseases of nonhematopoietic tissues in susceptible strains of mice. We have reported on the molecular cloning of a bone-tumorigenic virus, RFB-14 MuLV, which was found to induce benign bone tumors, osteomas, with 100% incidence in mice of the CBA/Ca strain (L. Pedersen, W. Behnisch, J. Schmidt, A. Luz, F. S. Pedersen, V. Erfle, and P. G. Strauss, J. Virol. 66:6186-6190, 1992). In order to analyze the bone tumor-inducing phenotype of RFB-14 MuLV, we have studied the pathogenic potential of recombinant viruses between RFB-14 and the nonosteomagenic, highly leukemogenic SL3-3 MuLV. The recombinants were constructed so as to reveal whether a major determinant of osteomagenicity maps to sequences within or outside the long terminal repeats (LTR). Our data show that a major determinant of the osteoma-inducing potential of RFB-14 MuLV maps to the non-LTR region of the genome. Furthermore, we demonstrate that a strong determinant of leukemogenicity is harbored by the non-LTR region of SL3-3 MuLV.
OriginalsprogEngelsk
TidsskriftJournal of Virology
Vol/bind71
Nummer1
Sider (fra-til)645-649
Antal sider5
ISSN0022-538X
StatusUdgivet - jan. 1997

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