Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Hossein Mohammad-Beigi, Yuya Hayashi, Christina Moeslund Zeuthen, Hoda Eskandari, Carsten Scavenius, Kristian Juul-Madsen, Thomas Vorup-Jensen, Jan J. Enghild, Duncan S Sutherland*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

147 Citationer (Scopus)

Abstract

The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

OriginalsprogEngelsk
Artikelnummer4535
TidsskriftNature Communications
Vol/bind11
Nummer1
Antal sider16
ISSN2041-1723
DOI
StatusUdgivet - 1 dec. 2020

Fingeraftryk

Dyk ned i forskningsemnerne om 'Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association'. Sammen danner de et unikt fingeraftryk.

Citationsformater