TY - JOUR
T1 - Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association
AU - Mohammad-Beigi, Hossein
AU - Hayashi, Yuya
AU - Zeuthen, Christina Moeslund
AU - Eskandari, Hoda
AU - Scavenius, Carsten
AU - Juul-Madsen, Kristian
AU - Vorup-Jensen, Thomas
AU - Enghild, Jan J.
AU - Sutherland, Duncan S
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.
AB - The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.
UR - http://www.scopus.com/inward/record.url?scp=85090816701&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-18237-7
DO - 10.1038/s41467-020-18237-7
M3 - Journal article
C2 - 32913217
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4535
ER -