Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis

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Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. / Wang, Yaping; Elhiti, Mohamed; Hebelstrup, Kim; Hill, Robert D; Stasolla, Claudio.

I: Plant Physiology and Biochemistry, Bind 49, Nr. 10, 10.2011, s. 1108-1116.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Wang, Y, Elhiti, M, Hebelstrup, K, Hill, RD & Stasolla, C 2011, 'Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis' Plant Physiology and Biochemistry, bind 49, nr. 10, s. 1108-1116. https://doi.org/10.1016/j.plaphy.2011.06.005

APA

Wang, Y., Elhiti, M., Hebelstrup, K., Hill, R. D., & Stasolla, C. (2011). Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. Plant Physiology and Biochemistry, 49(10), 1108-1116. https://doi.org/10.1016/j.plaphy.2011.06.005

CBE

Wang Y, Elhiti M, Hebelstrup K, Hill RD, Stasolla C. 2011. Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. Plant Physiology and Biochemistry. 49(10):1108-1116. https://doi.org/10.1016/j.plaphy.2011.06.005

MLA

Vancouver

Wang Y, Elhiti M, Hebelstrup K, Hill RD, Stasolla C. Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. Plant Physiology and Biochemistry. 2011 okt;49(10):1108-1116. https://doi.org/10.1016/j.plaphy.2011.06.005

Author

Wang, Yaping ; Elhiti, Mohamed ; Hebelstrup, Kim ; Hill, Robert D ; Stasolla, Claudio. / Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis. I: Plant Physiology and Biochemistry. 2011 ; Bind 49, Nr. 10. s. 1108-1116.

Bibtex

@article{c304896ca88c49be993b679b967f358e,
title = "Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis",
abstract = "Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role of hemoglobins during invitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed by culturing root explants on an initial auxin-rich callus induction medium (CIM) followed by a transfer onto a cytokinin-containing shoot induction medium (SIM). While the repression of GLB2 inhibited organogenesis the over-expression of GLB1 or GLB2 enhanced the number of shoots produced in culture, and altered the transcript levels of genes participating in cytokinin perception and signalling. The up-regulation of GLB1 or GLB2 activated CKI1 and AHK3, genes encoding cytokinin receptors and affected the transcript levels of cytokinin responsive regulators (ARRs). The expression of Type-A ARRs (ARR4, 5, 7, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants to cytokinin allowing the 35S::GLB1 and 35S::GLB2 lines to produce shoots at low cytokinin concentrations which did not promote organogenesis in the WT line. These results show that manipulation of hemoglobin can modify shoot organogenesis in Arabidopsis and possibly in those systems partially or completely unresponsive to applications of exogenous cytokinins.",
author = "Yaping Wang and Mohamed Elhiti and Kim Hebelstrup and Hill, {Robert D} and Claudio Stasolla",
year = "2011",
month = "10",
doi = "10.1016/j.plaphy.2011.06.005",
language = "English",
volume = "49",
pages = "1108--1116",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
publisher = "Elsevier Masson",
number = "10",

}

RIS

TY - JOUR

T1 - Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis

AU - Wang, Yaping

AU - Elhiti, Mohamed

AU - Hebelstrup, Kim

AU - Hill, Robert D

AU - Stasolla, Claudio

PY - 2011/10

Y1 - 2011/10

N2 - Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role of hemoglobins during invitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed by culturing root explants on an initial auxin-rich callus induction medium (CIM) followed by a transfer onto a cytokinin-containing shoot induction medium (SIM). While the repression of GLB2 inhibited organogenesis the over-expression of GLB1 or GLB2 enhanced the number of shoots produced in culture, and altered the transcript levels of genes participating in cytokinin perception and signalling. The up-regulation of GLB1 or GLB2 activated CKI1 and AHK3, genes encoding cytokinin receptors and affected the transcript levels of cytokinin responsive regulators (ARRs). The expression of Type-A ARRs (ARR4, 5, 7, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants to cytokinin allowing the 35S::GLB1 and 35S::GLB2 lines to produce shoots at low cytokinin concentrations which did not promote organogenesis in the WT line. These results show that manipulation of hemoglobin can modify shoot organogenesis in Arabidopsis and possibly in those systems partially or completely unresponsive to applications of exogenous cytokinins.

AB - Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role of hemoglobins during invitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed by culturing root explants on an initial auxin-rich callus induction medium (CIM) followed by a transfer onto a cytokinin-containing shoot induction medium (SIM). While the repression of GLB2 inhibited organogenesis the over-expression of GLB1 or GLB2 enhanced the number of shoots produced in culture, and altered the transcript levels of genes participating in cytokinin perception and signalling. The up-regulation of GLB1 or GLB2 activated CKI1 and AHK3, genes encoding cytokinin receptors and affected the transcript levels of cytokinin responsive regulators (ARRs). The expression of Type-A ARRs (ARR4, 5, 7, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants to cytokinin allowing the 35S::GLB1 and 35S::GLB2 lines to produce shoots at low cytokinin concentrations which did not promote organogenesis in the WT line. These results show that manipulation of hemoglobin can modify shoot organogenesis in Arabidopsis and possibly in those systems partially or completely unresponsive to applications of exogenous cytokinins.

U2 - 10.1016/j.plaphy.2011.06.005

DO - 10.1016/j.plaphy.2011.06.005

M3 - Journal article

VL - 49

SP - 1108

EP - 1116

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

IS - 10

ER -