Macrophage-mediated nanoparticle delivery to the periodontal lesions in established murine model via Pg-LPS induction

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  • Zhiwei Ma, State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi-an, China.
  • ,
  • Frederik Dagnaes-Hansen, Danmark
  • Henrik Løvschall
  • Wen Song, Danmark
  • Gitte K Nielsen, Danmark
  • Chuanxu Yang, Danmark
  • Qintao Wang
  • ,
  • Jørgen Kjems
  • Shan Gao, Danmark

We established a murine periodontitis model by local injection of lipopolysaccharide of Porphyromonas gingivalis (Pg-LPS) into the gingival sulcus of mandibular left incisor four times with 48-h interval. The histological examination of the periodontal tissues demonstrated that significant loss of periodontal bone and ligaments was observed in the lesion side with abundant inflammatory cell infiltration. Two days after the last injection, Cy5-labelled siRNA/chitosan particles were injected intraperitoneally (ip). The chitosan/siRNA particles were taken up by peritoneal macrophages, which subsequently migrated to the inflamed gingival area evaluated by in vivo imaging. The localization of macrophages in the inflamed region was further confirmed by immunofluorescent staining. The present report demonstrates that intragingival injection of Pg-LPS can be used to create an experimental model of periodontal inflammation in mice and that recruitment of macrophages with chitosan/siRNA nanoparticles to the inflamed area opens the possibility of an RNAi-based therapeutic approach using chitosan as a carrier in periodontitis.

OriginalsprogEngelsk
TidsskriftJournal of Oral Pathology & Medicine
Vol/bind44
Nummer7
Sider (fra-til)538-542
Antal sider5
ISSN0904-2512
DOI
StatusUdgivet - aug. 2015

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