TY - JOUR
T1 - Lotus japonicus Genetic, Mutant, and Germplasm Resources
AU - Hashiguchi, Masatsugu
AU - Tanaka, Hidenori
AU - Muguerza, Melody
AU - Akashi, Ryo
AU - Sandal, Niels Nørgaard
AU - Andersen, Stig Uggerhøj
AU - Sato, Shusei
N1 - Copyright © 2018 John Wiley & Sons, Inc.
PY - 2018/6
Y1 - 2018/6
N2 - A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.
AB - A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.
KW - LORE1 lines
KW - Lotus japonicus
KW - genome information browser
KW - legume
KW - root culture system
KW - wild accessions
UR - http://www.scopus.com/inward/record.url?scp=85055914912&partnerID=8YFLogxK
U2 - 10.1002/cppb.20070
DO - 10.1002/cppb.20070
M3 - Journal article
C2 - 29927119
SN - 2379-8068
VL - 3
SP - e20070
JO - Current protocols in plant biology
JF - Current protocols in plant biology
IS - 2
M1 - e20070
ER -