Lotus japonicus ARPC1 is required for Rhizobial Infection

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  • Md Shakhawat Hossain, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Jinqiu Liao, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Euan K James, The James Hutton Institute, Storbritannien
  • Shusei Sato, The James Hutton Institute, Japan
  • Satoshi Tabata, The James Hutton Institute, Japan
  • Anna Jurkiewicz, Danmark
  • Lene Heegaard Madsen
  • Jens Stougaard
  • Loretta Ross, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Krzysztof Szczyglowski, Department of Biology, University of Western Ontario, Canada
Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nck-associated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry.
OriginalsprogEngelsk
TidsskriftPlant Physiology
Vol/bind160
Nummer2
Sider (fra-til)917-928
Antal sider12
ISSN0032-0889
DOI
StatusUdgivet - 3 aug. 2012

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