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Lipase-catalyzed acidolysis of canola oil with caprylic acid to produce medium-, long- and medium-chain-type structured lipids

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  • Yingyao Wang, Academy of State Administration of Grain, Kina
  • Luan Xia, Academy of State Administration of Grain, Kina
  • Xuebing Xu, Danmark
  • Liang Xie, Suzhou Ovodan Egg products Co, Kina
  • Zhangqun Duan, Academy of State Administration of Grain, Kina
Lipase-catalyzed acidolysis of canola oil with caprylic acid was performed to produce structured lipids (SLs) containing medium-chain fatty acid (M) at position sn-1,3 and long-chain fatty acid (L) at the sn-2 position in a solvent-free system. Six commercial lipases from different sources were screened for their ability to incorporate caprylic acid into the canola oil. The sn-1,3 regiospecificity toward the glycerol backbone of canola oil of the lipases with relatively higher acidolysis activity was compared by investigating the fatty acid profiles of the products. The results showed that Lipozyme RM IM from Rhizomucor miehei resulted in the highest caprylic acid incorporation ability and the lowest acyl migration. The reaction parameters including substrate mole ratio, enzyme load, reaction time and temperature of Lipozyme RM IM were investigated. Incorporation of caprylic acid was higher when reactions were carried with 10% lipase of the total weight of substrates with a 1:4 mole ratio of oil and caprylic acid. The optimal time course and temperature for synthesis SLs were 15 h and 50–60 °C. Possible triacylglycerol species and physical properties of the SLs product obtained at relative optimal conditions were characterized
OriginalsprogEngelsk
TidsskriftFood and Bioproducts Processing
Vol/bind90
Nummer4
Sider (fra-til)707-712
Antal sider5
ISSN0960-3085
DOI
StatusUdgivet - okt. 2012

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