TY - ICOMM
T1 - Labelling of Cells Engaged in DNA Synthesis: Autoradiography and BrdU Staining
AU - Madsen, Peder Søndergaard
PY - 2010/10/18
Y1 - 2010/10/18
N2 - The cell cycle is divided in four phases: G1 phase, S phase (DNA-synthesis), G2 phase (together termed interphase) and M phase (mitosis). Cells that have ceased proliferation enter a state of quiescence called G0. M phase is itself composed of two tightly coupled processes: mitosis, in which the cell's chromosomes are divided between the two daughter cells, and cytokinesis, in which the cell's cytoplasm divides in half forming distinct new cells. It is often necessary to evaluate the proportion of DNA-replicating nuclei (S-phase cells) in a given population of cells. Classical methods for detection of cells in the S phase use incorporation of modified nucleosides, followed by direct visualisation of the DNA -replicating cells
AB - The cell cycle is divided in four phases: G1 phase, S phase (DNA-synthesis), G2 phase (together termed interphase) and M phase (mitosis). Cells that have ceased proliferation enter a state of quiescence called G0. M phase is itself composed of two tightly coupled processes: mitosis, in which the cell's chromosomes are divided between the two daughter cells, and cytokinesis, in which the cell's cytoplasm divides in half forming distinct new cells. It is often necessary to evaluate the proportion of DNA-replicating nuclei (S-phase cells) in a given population of cells. Classical methods for detection of cells in the S phase use incorporation of modified nucleosides, followed by direct visualisation of the DNA -replicating cells
KW - cell cycle, DNA replication, cell proliferation, S phase, DNA synthesis, bromodeoxyuridine (BrdU), [3H]thymidine
U2 - 10.1002/9780470015902.a0002572.pub2
DO - 10.1002/9780470015902.a0002572.pub2
M3 - Net publication - Internet publication
ER -