Isolation of an alfalfa histone H3 gene: structure and expression

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Standard

Isolation of an alfalfa histone H3 gene: structure and expression. / Wu, S-C; Bögre, L; Vincze, Éva; Kiss, G B; Dudits, D.

I: Plant Molecular Biology, Bind 11, 1988, s. 641-649.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Wu, S-C, Bögre, L, Vincze, É, Kiss, GB & Dudits, D 1988, 'Isolation of an alfalfa histone H3 gene: structure and expression', Plant Molecular Biology, bind 11, s. 641-649. https://doi.org/10.1007/BF00017464

APA

Wu, S-C., Bögre, L., Vincze, É., Kiss, G. B., & Dudits, D. (1988). Isolation of an alfalfa histone H3 gene: structure and expression. Plant Molecular Biology, 11, 641-649. https://doi.org/10.1007/BF00017464

CBE

Wu S-C, Bögre L, Vincze É, Kiss GB, Dudits D. 1988. Isolation of an alfalfa histone H3 gene: structure and expression. Plant Molecular Biology. 11:641-649. https://doi.org/10.1007/BF00017464

MLA

Vancouver

Wu S-C, Bögre L, Vincze É, Kiss GB, Dudits D. Isolation of an alfalfa histone H3 gene: structure and expression. Plant Molecular Biology. 1988;11:641-649. https://doi.org/10.1007/BF00017464

Author

Wu, S-C ; Bögre, L ; Vincze, Éva ; Kiss, G B ; Dudits, D. / Isolation of an alfalfa histone H3 gene: structure and expression. I: Plant Molecular Biology. 1988 ; Bind 11. s. 641-649.

Bibtex

@article{baa35660ac0a11dda608000ea68e967b,
title = "Isolation of an alfalfa histone H3 gene: structure and expression",
abstract = "A histone H3 gene was isolated from a dicotyledonous plant, alfalfa (Medicago sativa). The sequence analysis of this gene revealed no obvious GC preference in its codon usage. Apart from containing most of the typical consensus sequences found in both animal and plant histone genes, the alfalfa H3 gene exhibits distinct structural features such as (1) the unusual location of two GATCC motifs in its 5' flanking sequence, (2) the existence of a CGCGGATC on the nonsense strand at position –232, (3) the existence of a long palindromic structure, and (4) several polyadenylation signal-like sequences in the 3' flanking region. There are about 160 copies of histone H3 gene in alfalfa tetraploid genome. Using the alfalfa H3 gene as a probe to study the pattern of histone H3 transcripts in the alfalfa, we found that the H3 RNAs are undetectable in leaves, more in stems than in roots, and highest in somatic embryos. Moreover, the RNA products of H3 genes in all alfalfa tissues tested show unusually long nontranslated region compared to those of animal histone genes. An additional high molecular weight species of H3 transcript was detected only in somatic embryos.",
author = "S-C Wu and L B{\"o}gre and {\'E}va Vincze and Kiss, {G B} and D Dudits",
year = "1988",
doi = "10.1007/BF00017464",
language = "English",
volume = "11",
pages = "641--649",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Isolation of an alfalfa histone H3 gene: structure and expression

AU - Wu, S-C

AU - Bögre, L

AU - Vincze, Éva

AU - Kiss, G B

AU - Dudits, D

PY - 1988

Y1 - 1988

N2 - A histone H3 gene was isolated from a dicotyledonous plant, alfalfa (Medicago sativa). The sequence analysis of this gene revealed no obvious GC preference in its codon usage. Apart from containing most of the typical consensus sequences found in both animal and plant histone genes, the alfalfa H3 gene exhibits distinct structural features such as (1) the unusual location of two GATCC motifs in its 5' flanking sequence, (2) the existence of a CGCGGATC on the nonsense strand at position –232, (3) the existence of a long palindromic structure, and (4) several polyadenylation signal-like sequences in the 3' flanking region. There are about 160 copies of histone H3 gene in alfalfa tetraploid genome. Using the alfalfa H3 gene as a probe to study the pattern of histone H3 transcripts in the alfalfa, we found that the H3 RNAs are undetectable in leaves, more in stems than in roots, and highest in somatic embryos. Moreover, the RNA products of H3 genes in all alfalfa tissues tested show unusually long nontranslated region compared to those of animal histone genes. An additional high molecular weight species of H3 transcript was detected only in somatic embryos.

AB - A histone H3 gene was isolated from a dicotyledonous plant, alfalfa (Medicago sativa). The sequence analysis of this gene revealed no obvious GC preference in its codon usage. Apart from containing most of the typical consensus sequences found in both animal and plant histone genes, the alfalfa H3 gene exhibits distinct structural features such as (1) the unusual location of two GATCC motifs in its 5' flanking sequence, (2) the existence of a CGCGGATC on the nonsense strand at position –232, (3) the existence of a long palindromic structure, and (4) several polyadenylation signal-like sequences in the 3' flanking region. There are about 160 copies of histone H3 gene in alfalfa tetraploid genome. Using the alfalfa H3 gene as a probe to study the pattern of histone H3 transcripts in the alfalfa, we found that the H3 RNAs are undetectable in leaves, more in stems than in roots, and highest in somatic embryos. Moreover, the RNA products of H3 genes in all alfalfa tissues tested show unusually long nontranslated region compared to those of animal histone genes. An additional high molecular weight species of H3 transcript was detected only in somatic embryos.

U2 - 10.1007/BF00017464

DO - 10.1007/BF00017464

M3 - Journal article

VL - 11

SP - 641

EP - 649

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

ER -