Isolation and characterization of an R prime plasmid in Rhizobium meliloti

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • G B Kiss, Danmark
  • K Dobó, Danmark
  • I Dusha, Danmark
  • A Breznovits, Danmark
  • L Orosz, Danmark
  • Éva Vincze
  • A Kondorosi, Danmark
Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria
OriginalsprogEngelsk
TidsskriftJournal of Bacteriology
Vol/bind141
Sider (fra-til)121-128
Antal sider8
ISSN0021-9193
StatusUdgivet - 1980
Eksternt udgivetJa

Se relationer på Aarhus Universitet Citationsformater

ID: 1488479