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Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening.

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskning

  • Lone Vedel Schøler, Danmark
  • Tine Hørning Møller, Danmark
  • Steffen Nørgaard, Institut for Molekylærbiologi og Genetik, Danmark
  • Lotte Vestergård
  • Anders Olsen, Danmark
The soil nematode Caenorhabditis elegans has become a popular genetic model organism used to study a broad range of complex biological processes, including development, aging, apoptosis, and DNA damage responses. Many genetic tools and tricks have been developed in C. elegans including knock down of gene expression via RNA interference (RNAi). In C. elegans RNAi can effectively be administrated via feeding the nematodes bacteria expressing double-stranded RNA targeting the gene of interest. Several commercial C. elegans RNAi libraries are available and hence gene inactivation using RNAi can relatively easily be performed in a genome-wide fashion. In this chapter we give a protocol for using genome-wide RNAi screening to identify genes involved with the response to genotoxic stress.
OriginalsprogEngelsk
TitelDNA Repair Protocols : Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening
RedaktørerLotte Bjergbæk
Antal sider12
Vol/bind920
UdgivelsesstedNew York
ForlagSpringer Science+Business Media
Udgivelsesår2012
Udgave1
Sider27-38
Kapitel3
ISBN (trykt)978-1-61779-997-6
ISBN (Elektronisk)978-1-61779-998-3
DOI
StatusUdgivet - 2012
SerietitelMethods in Molecular Biology
ISSN1064-3745

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