TY - JOUR
T1 - Serratia inhibens sp. nov., a new antifungal species isolated from potato (solanum tuberosum)
AU - Hennessy, Rosanna C.
AU - Dichmann, Søs I.
AU - Martens, Helle Juel
AU - Zervas, Athanasios
AU - Stougaard, Peter
PY - 2020/6
Y1 - 2020/6
N2 - A novel bacterial strain, S40T, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40T. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated gyrB, rpoD, infB and atpD sequences) showed that strain S40T was affiliated with the genus Serratia and with Serratia plymuthica PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26%; DNA–DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40T and S. plymuthica PRI-2C genomes displayed lower similarities when compared to all other S. plymuthica strains (ANI ≤94.34%; dDDH ≤57.6% relatedness). The DNA G+C content of strain S40T was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10–37 °C (optimum, 25–30 °C) and at pH 6–9 (optimum, pH 6–7). Major fatty acids were C16:0 (27.9%), summed feature (C16:1 ω6c/C16:1 ω7c; 18.0%) and C17:0 cyclo (15.1%). The respiratory quinone was determined to be Q8 (94%) and MK8 (95%) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40T represents a novel species of the genus Serratia, for which the name Serratia inhibens sp. nov. is proposed. The type strain is S40T (=LMG 31467T=NCIMB 15235T). In addition, we propose that S. plymuthica PRI-2C is reclassified and transferred to the species S. inhibens as S. inhibens PRI-2C.
AB - A novel bacterial strain, S40T, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40T. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated gyrB, rpoD, infB and atpD sequences) showed that strain S40T was affiliated with the genus Serratia and with Serratia plymuthica PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26%; DNA–DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40T and S. plymuthica PRI-2C genomes displayed lower similarities when compared to all other S. plymuthica strains (ANI ≤94.34%; dDDH ≤57.6% relatedness). The DNA G+C content of strain S40T was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10–37 °C (optimum, 25–30 °C) and at pH 6–9 (optimum, pH 6–7). Major fatty acids were C16:0 (27.9%), summed feature (C16:1 ω6c/C16:1 ω7c; 18.0%) and C17:0 cyclo (15.1%). The respiratory quinone was determined to be Q8 (94%) and MK8 (95%) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40T represents a novel species of the genus Serratia, for which the name Serratia inhibens sp. nov. is proposed. The type strain is S40T (=LMG 31467T=NCIMB 15235T). In addition, we propose that S. plymuthica PRI-2C is reclassified and transferred to the species S. inhibens as S. inhibens PRI-2C.
KW - ANI
KW - Antimicrobial activity
KW - DDDH
KW - Potato
KW - Serratia
KW - Whole genome analysis
UR - http://www.scopus.com/inward/record.url?scp=85088880378&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004270
DO - 10.1099/ijsem.0.004270
M3 - Journal article
C2 - 32553053
AN - SCOPUS:85088880378
SN - 1466-5026
VL - 70
SP - 4204
EP - 4211
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 7
M1 - 004270
ER -