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Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung

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Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung. / Mühlfeld, Christian; Weibel, Ewald R.; Hahn, Ute; Kummer, Wolfgang; Nyengaard, Jens Randel; Ochs, Matthias.

I: Anatomical Record, Bind 293, Nr. 7, 2010, s. 1270-1275.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Mühlfeld, Christian ; Weibel, Ewald R. ; Hahn, Ute ; Kummer, Wolfgang ; Nyengaard, Jens Randel ; Ochs, Matthias. / Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung. I: Anatomical Record. 2010 ; Bind 293, Nr. 7. s. 1270-1275.

Bibtex

@article{77f9d710129a11dfb95d000ea68e967b,
title = "Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung",
abstract = "Stereological estimations of total capillary length have been used to characterize changes in the alveolar capillary network (ACN) during developmental processes or pathophysiological conditions. Here, we analyzed whether length estimations are appropriate to describe the 3D nature of the ACN. Semi-thin sections of five human lungs, previously investigated by Gehr et al. (Respir Physiol 1978; 32:121–140), were used to estimate alveolar capillary length using a “design-based” or a “model-based” stereological approach. The design-based approach involves counting of capillary profiles related to a defined area of the reference space. The model-based approach bases on the assumption that capillaries are round tubes and length was calculated from capillary volume and surface area. The model-based approach provided a mean of 6,950 km (SD: 3,108 km) for total capillary length, the design-based approach resulted in a mean of 2,746 km (SD: 722 km). Because of the geometry of the ACN both approaches carry an unpredictable bias. The bias incurred by the design-based approach is proportional to the ratio between radius and length of the capillary segments in the ACN, the number of branching points and the winding of the capillaries. The model-based approach is biased because of the real noncylindrical shape of capillaries and the network structure. In conclusion, the estimation of the total length of capillaries in the ACN cannot be recommended as the geometry of the ACN does not fulfill the requirements for stereological length estimation. Until new methods are being developed, the unbiased estimates of capillary volume, and surface area should be preferred.",
author = "Christian M{\"u}hlfeld and Weibel, {Ewald R.} and Ute Hahn and Wolfgang Kummer and Nyengaard, {Jens Randel} and Matthias Ochs",
year = "2010",
doi = "10.1002/ar.21158",
language = "English",
volume = "293",
pages = "1270--1275",
journal = "Anatomical Record",
issn = "1932-8486",
publisher = "Wiley",
number = "7",

}

RIS

TY - JOUR

T1 - Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung

AU - Mühlfeld, Christian

AU - Weibel, Ewald R.

AU - Hahn, Ute

AU - Kummer, Wolfgang

AU - Nyengaard, Jens Randel

AU - Ochs, Matthias

PY - 2010

Y1 - 2010

N2 - Stereological estimations of total capillary length have been used to characterize changes in the alveolar capillary network (ACN) during developmental processes or pathophysiological conditions. Here, we analyzed whether length estimations are appropriate to describe the 3D nature of the ACN. Semi-thin sections of five human lungs, previously investigated by Gehr et al. (Respir Physiol 1978; 32:121–140), were used to estimate alveolar capillary length using a “design-based” or a “model-based” stereological approach. The design-based approach involves counting of capillary profiles related to a defined area of the reference space. The model-based approach bases on the assumption that capillaries are round tubes and length was calculated from capillary volume and surface area. The model-based approach provided a mean of 6,950 km (SD: 3,108 km) for total capillary length, the design-based approach resulted in a mean of 2,746 km (SD: 722 km). Because of the geometry of the ACN both approaches carry an unpredictable bias. The bias incurred by the design-based approach is proportional to the ratio between radius and length of the capillary segments in the ACN, the number of branching points and the winding of the capillaries. The model-based approach is biased because of the real noncylindrical shape of capillaries and the network structure. In conclusion, the estimation of the total length of capillaries in the ACN cannot be recommended as the geometry of the ACN does not fulfill the requirements for stereological length estimation. Until new methods are being developed, the unbiased estimates of capillary volume, and surface area should be preferred.

AB - Stereological estimations of total capillary length have been used to characterize changes in the alveolar capillary network (ACN) during developmental processes or pathophysiological conditions. Here, we analyzed whether length estimations are appropriate to describe the 3D nature of the ACN. Semi-thin sections of five human lungs, previously investigated by Gehr et al. (Respir Physiol 1978; 32:121–140), were used to estimate alveolar capillary length using a “design-based” or a “model-based” stereological approach. The design-based approach involves counting of capillary profiles related to a defined area of the reference space. The model-based approach bases on the assumption that capillaries are round tubes and length was calculated from capillary volume and surface area. The model-based approach provided a mean of 6,950 km (SD: 3,108 km) for total capillary length, the design-based approach resulted in a mean of 2,746 km (SD: 722 km). Because of the geometry of the ACN both approaches carry an unpredictable bias. The bias incurred by the design-based approach is proportional to the ratio between radius and length of the capillary segments in the ACN, the number of branching points and the winding of the capillaries. The model-based approach is biased because of the real noncylindrical shape of capillaries and the network structure. In conclusion, the estimation of the total length of capillaries in the ACN cannot be recommended as the geometry of the ACN does not fulfill the requirements for stereological length estimation. Until new methods are being developed, the unbiased estimates of capillary volume, and surface area should be preferred.

U2 - 10.1002/ar.21158

DO - 10.1002/ar.21158

M3 - Journal article

C2 - 20583281

VL - 293

SP - 1270

EP - 1275

JO - Anatomical Record

JF - Anatomical Record

SN - 1932-8486

IS - 7

ER -