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Iron corrosion by methanogenic archaea characterized by stable isotope effects and crust mineralogy

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  • Marc Tamisier, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Matthias Schmidt, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Carsten Vogt, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Steffen Kümmel, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Hryhoriy Stryhanyuk, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Niculina Musat
  • Hans Hermann Richnow, Helmholtz-Gemeinschaft Deutscher Forschungszentren
  • ,
  • Florin Musat

Carbon and hydrogen stable isotope effects associated with methane formation by the corrosive archaeon Methanobacterium strain IM1 were determined during growth with hydrogen and iron. Isotope analyses were complemented by structural, elemental and molecular composition analyses of corrosion crusts. During growth with H2, strain IM1 formed methane with average δ13C of −43.5‰ and δ2H of −370‰. Corrosive growth led to methane more depleted in 13C, with average δ13C ranging from −56‰ to −64‰ during the early and the late growth phase respectively. The corresponding δ2H were less impacted by the growth phase, with average values ranging from −316 to −329‰. The stable isotope fractionation factors, (Formula presented.), were 1.026 and 1.042 for hydrogenotrophic and corrosive growth respectively. Corrosion crusts formed by strain IM1 have a domed structure, appeared electrically conductive and were composed of siderite, calcite and iron sulfide, the latter formed by precipitation of sulfide (from culture medium) with ferrous iron generated during corrosion. Strain IM1 cells were found attached to crust surfaces and encrusted deep inside crust domes. Our results may assist to diagnose methanogens-induced corrosion in the field and suggest that intrusion of sulfide in anoxic settings may stimulate corrosion by methanogenic archaea via formation of semiconductive crusts.

TidsskriftEnvironmental Microbiology
Sider (fra-til)583-595
Antal sider13
StatusUdgivet - feb. 2022
Eksternt udgivetJa

Bibliografisk note

Publisher Copyright:
© 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

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