Investigation of Synaptic Vesicle Proteins in Rat Brain Tissue Using Real-Time qPCR

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For many years real-time quantitative polymerase chain reaction (qPCR) has been the golden standard to measure gene expression levels in brain tissue. However, today it is generally accepted that many factors may affect the outcome of the study and more consensus is required to perform and interpret real-time qPCR experiments in a comparable way. Here we describe the basic techniques used for more than a decade in our laboratory to extract RNA and protein from the same piece of frozen brain tissue and to quantify relative mRNA levels with real-time qPCR and SYBR Green.

OriginalsprogEngelsk
TitelSynaptic Vesicles : Methods and Protocols
RedaktørerJ Dahlmanns, M Dahlmanns
Antal sider10
ForlagSpringer
Udgivelsesår2022
Sider59-68
ISBN (trykt)978-1-0716-1916-2
DOI
StatusUdgivet - 2022
SerietitelMethods in Molecular Biology
Vol/bind2417
ISSN1064-3745

Bibliografisk note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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