TY - JOUR
T1 - Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland
AU - Thøgersen, Mariane Schmidt
AU - Zervas, Athanasios
AU - Stougaard, Peter
AU - Ellegaard-Jensen, Lea
N1 - Publisher Copyright:
Copyright © 2024 Thøgersen, Zervas, Stougaard and Ellegaard-Jensen.
PY - 2024/4
Y1 - 2024/4
N2 - The ikaite columns in the Ikka Fjord, SW Greenland, represent a permanently cold and alkaline environment known to contain a rich bacterial diversity. 16S and 18S rRNA gene amplicon and metagenomic sequencing was used to investigate the microbial diversity in the columns and for the first time, the eukaryotic and archaeal diversity in ikaite columns were analyzed. The results showed a rich prokaryotic diversity that varied across columns as well as within each column. Seven different archaeal phyla were documented in multiple locations inside the columns. The columns also contained a rich eukaryotic diversity with 27 phyla representing microalgae, protists, fungi, and small animals. Based on metagenomic sequencing, 25 high-quality MAGs were assembled and analyzed for the presence of genes involved in cycling of nitrogen, sulfur, and phosphorous as well as genes encoding carbohydrate-active enzymes (CAZymes), showing a potentially very bioactive microbial community.
AB - The ikaite columns in the Ikka Fjord, SW Greenland, represent a permanently cold and alkaline environment known to contain a rich bacterial diversity. 16S and 18S rRNA gene amplicon and metagenomic sequencing was used to investigate the microbial diversity in the columns and for the first time, the eukaryotic and archaeal diversity in ikaite columns were analyzed. The results showed a rich prokaryotic diversity that varied across columns as well as within each column. Seven different archaeal phyla were documented in multiple locations inside the columns. The columns also contained a rich eukaryotic diversity with 27 phyla representing microalgae, protists, fungi, and small animals. Based on metagenomic sequencing, 25 high-quality MAGs were assembled and analyzed for the presence of genes involved in cycling of nitrogen, sulfur, and phosphorous as well as genes encoding carbohydrate-active enzymes (CAZymes), showing a potentially very bioactive microbial community.
KW - biodiversity
KW - biotechnological potential
KW - eukaryotes
KW - Greenland
KW - ikaite columns
KW - metagenome
KW - prokaryotes
UR - http://www.scopus.com/inward/record.url?scp=85190837114&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2024.1358787
DO - 10.3389/fmicb.2024.1358787
M3 - Journal article
C2 - 38655082
AN - SCOPUS:85190837114
SN - 1664-302X
VL - 15
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1358787
ER -