Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling

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Chemically modified antigen-binding proteins are widely applied for their targeting abilities in the fields of biotechnology, medicine, and diagnostics. However, the production of site-selectively modified proteins remains a challenge. Here, we have designed a chemical probe for the introduction of a reactive aldehyde on nanobodies by metal-complex-guided conjugation. The probe design allows for purification of the conjugates, and the aldehyde constitutes an efficient handle for further modification of the nanobodies. In vitro experiments confirmed the binding activity and selectivity of fluorescent conjugates toward the native antigen. Furthermore, the modification strategy allowed for production of a nanobody-drug conjugate that was active in vitro.

OriginalsprogEngelsk
TidsskriftBioconjugate Chemistry
Vol/bind31
Nummer5
Sider (fra-til)1295-1300
Antal sider6
ISSN1043-1802
DOI
StatusUdgivet - maj 2020

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