Aarhus University Seal / Aarhus Universitets segl

Intracellular Delivery of a Planar DNA Origami Structure by the Transferrin-Receptor Internalization Pathway

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review


  • David Henning Schaffert
  • ,
  • Anders Hauge Okholm
  • ,
  • Rasmus Schøler Sørensen
  • ,
  • Jesper Nielsen, Institut for Molekylærbiologi og Genetik
  • ,
  • Thomas Tørring
  • Christian Bech Rosen
  • ,
  • Anne Louise Bank Kodal
  • ,
  • Michael Rosholm Mortensen
  • ,
  • Kurt Vesterager Gothelf
  • Jørgen Kjems
DNA origami provides rapid access to easily functionalized, nanometer-sized structures making it an intriguing platform for the development of defined drug delivery and sensor systems. Low cellular uptake of DNA nanostructures is a major obstacle in the development of DNA-based delivery platforms. Herein, significant strong increase in cellular uptake in an established cancer cell line by modifying a planar DNA origami structure with the iron transport protein transferrin (Tf) is demonstrated. A variable number of Tf molecules are coupled to the origami structure using a DNA-directed, site-selective labeling technique to retain ligand functionality. A combination of confocal fluorescence microscopy and quantitative (qPCR) techniques shows up to 22-fold increased cytoplasmic uptake compared to unmodified structures and with an efficiency that correlates to the number of transferrin molecules on the origami surface.
Sider (fra-til)2634-2640
StatusUdgivet - 18 maj 2016

Se relationer på Aarhus Universitet Citationsformater

ID: 99739598