@article{baef21f8e4b340638cd959646c71ee97,
title = "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples",
abstract = "Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplified16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.",
keywords = "SOIL BACTERIAL, RIBOSOMAL-RNA, PLASMID DNA, ADSORPTION, COMMUNITY, COEXTRACTION, GENE",
author = "Jacobsen, {Carsten Suhr} and Nielsen, {Tue Kjaergaard} and Vester, {Jan Kjolhede} and Peter Stougaard and Nielsen, {Jeppe Lund} and Jana Voriskova and Anne Winding and Petr Baldrian and Binbin Liu and Asa Frostegard and Dorthe Pedersen and Tveit, {Alexander Tosdal} and Svenning, {Mette Marianne} and Tebbe, {Christoph C.} and Lise Ovreas and Jakobsen, {Pia Bach} and Blazewicz, {Steven J.} and Valerie Hubablek and Stefan Bertilsson and Hansen, {Lars Hestbjerg} and Cary, {S. Craig} and Holben, {William E.} and Flemming Ekelund and Jacob Baelum",
year = "2018",
month = apr,
day = "9",
doi = "10.1038/s41598-018-24082-y",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}