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Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1

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Standard

Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. / Barra, Jasmine; Gaidos, Gabriel S.; Blumenthal, Ezra; Beckedorff, Felipe; Tayar, Mina M.; Kirstein, Nina; Karakac, Tobias K.; Jensen, Torben Heick; Impens, Francis; Gevaert, Kris; Leucci, Eleonora; Shiekhattar, Ramin; Marine, Jean Christophe.

I: Science Advances, Bind 6, Nr. 27, eaaz9072, 2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Barra, J, Gaidos, GS, Blumenthal, E, Beckedorff, F, Tayar, MM, Kirstein, N, Karakac, TK, Jensen, TH, Impens, F, Gevaert, K, Leucci, E, Shiekhattar, R & Marine, JC 2020, 'Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1', Science Advances, bind 6, nr. 27, eaaz9072. https://doi.org/10.1126/sciadv.aaz9072

APA

Barra, J., Gaidos, G. S., Blumenthal, E., Beckedorff, F., Tayar, M. M., Kirstein, N., Karakac, T. K., Jensen, T. H., Impens, F., Gevaert, K., Leucci, E., Shiekhattar, R., & Marine, J. C. (2020). Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Science Advances, 6(27), [eaaz9072]. https://doi.org/10.1126/sciadv.aaz9072

CBE

Barra J, Gaidos GS, Blumenthal E, Beckedorff F, Tayar MM, Kirstein N, Karakac TK, Jensen TH, Impens F, Gevaert K, Leucci E, Shiekhattar R, Marine JC. 2020. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Science Advances. 6(27):Article eaaz9072. https://doi.org/10.1126/sciadv.aaz9072

MLA

Vancouver

Barra J, Gaidos GS, Blumenthal E, Beckedorff F, Tayar MM, Kirstein N o.a. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Science Advances. 2020;6(27). eaaz9072. https://doi.org/10.1126/sciadv.aaz9072

Author

Barra, Jasmine ; Gaidos, Gabriel S. ; Blumenthal, Ezra ; Beckedorff, Felipe ; Tayar, Mina M. ; Kirstein, Nina ; Karakac, Tobias K. ; Jensen, Torben Heick ; Impens, Francis ; Gevaert, Kris ; Leucci, Eleonora ; Shiekhattar, Ramin ; Marine, Jean Christophe. / Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. I: Science Advances. 2020 ; Bind 6, Nr. 27.

Bibtex

@article{7ffecbcd82624dfd9b5ab209ee3191e0,
title = "Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1",
abstract = "RNA 3' end processing provides a source of transcriptome diversification which affects various (patho)-physiological processes. A prime example is the transcript isoform switch that leads to the read-through expression of the long non-coding RNA NEAT1_2, at the expense of the shorter polyadenylated transcript NEAT1_1. NEAT1_2 is required for assembly of paraspeckles (PS), nuclear bodies that protect cancer cells from oncogene-induced replication stress and chemotherapy. Searching for proteins that modulate this event, we identified factors involved in the 3' end processing of polyadenylated RNA and components of the Integrator complex. Perturbation experiments established that, by promoting the cleavage of NEAT1_2, Integrator forces NEAT1_2 to NEAT1_1 isoform switching and, thereby, restrains PS assembly. Consistently, low levels of Integrator subunits correlated with poorer prognosis of cancer patients exposed to chemotherapeutics. Our study establishes that Integrator regulates PS biogenesis and a link between Integrator, cancer biology, and chemosensitivity, which may be exploited therapeutically.",
author = "Jasmine Barra and Gaidos, {Gabriel S.} and Ezra Blumenthal and Felipe Beckedorff and Tayar, {Mina M.} and Nina Kirstein and Karakac, {Tobias K.} and Jensen, {Torben Heick} and Francis Impens and Kris Gevaert and Eleonora Leucci and Ramin Shiekhattar and Marine, {Jean Christophe}",
year = "2020",
doi = "10.1126/sciadv.aaz9072",
language = "English",
volume = "6",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "27",

}

RIS

TY - JOUR

T1 - Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1

AU - Barra, Jasmine

AU - Gaidos, Gabriel S.

AU - Blumenthal, Ezra

AU - Beckedorff, Felipe

AU - Tayar, Mina M.

AU - Kirstein, Nina

AU - Karakac, Tobias K.

AU - Jensen, Torben Heick

AU - Impens, Francis

AU - Gevaert, Kris

AU - Leucci, Eleonora

AU - Shiekhattar, Ramin

AU - Marine, Jean Christophe

PY - 2020

Y1 - 2020

N2 - RNA 3' end processing provides a source of transcriptome diversification which affects various (patho)-physiological processes. A prime example is the transcript isoform switch that leads to the read-through expression of the long non-coding RNA NEAT1_2, at the expense of the shorter polyadenylated transcript NEAT1_1. NEAT1_2 is required for assembly of paraspeckles (PS), nuclear bodies that protect cancer cells from oncogene-induced replication stress and chemotherapy. Searching for proteins that modulate this event, we identified factors involved in the 3' end processing of polyadenylated RNA and components of the Integrator complex. Perturbation experiments established that, by promoting the cleavage of NEAT1_2, Integrator forces NEAT1_2 to NEAT1_1 isoform switching and, thereby, restrains PS assembly. Consistently, low levels of Integrator subunits correlated with poorer prognosis of cancer patients exposed to chemotherapeutics. Our study establishes that Integrator regulates PS biogenesis and a link between Integrator, cancer biology, and chemosensitivity, which may be exploited therapeutically.

AB - RNA 3' end processing provides a source of transcriptome diversification which affects various (patho)-physiological processes. A prime example is the transcript isoform switch that leads to the read-through expression of the long non-coding RNA NEAT1_2, at the expense of the shorter polyadenylated transcript NEAT1_1. NEAT1_2 is required for assembly of paraspeckles (PS), nuclear bodies that protect cancer cells from oncogene-induced replication stress and chemotherapy. Searching for proteins that modulate this event, we identified factors involved in the 3' end processing of polyadenylated RNA and components of the Integrator complex. Perturbation experiments established that, by promoting the cleavage of NEAT1_2, Integrator forces NEAT1_2 to NEAT1_1 isoform switching and, thereby, restrains PS assembly. Consistently, low levels of Integrator subunits correlated with poorer prognosis of cancer patients exposed to chemotherapeutics. Our study establishes that Integrator regulates PS biogenesis and a link between Integrator, cancer biology, and chemosensitivity, which may be exploited therapeutically.

UR - http://www.scopus.com/inward/record.url?scp=85088434976&partnerID=8YFLogxK

U2 - 10.1126/sciadv.aaz9072

DO - 10.1126/sciadv.aaz9072

M3 - Journal article

AN - SCOPUS:85088434976

VL - 6

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 27

M1 - eaaz9072

ER -