Integrated transcriptomics and proteomics analysis of storage protein composition in developing barley grain to improve nutritional profile

Publikation: Bidrag til bog/antologi/rapport/proceedingKonferencebidrag i proceedingsForskning

  • Agnieszka Ewa Kaczmarczyk, Danmark
  • Giuseppe Dionisio
  • Jenny Renaut, Department of Environment and Agrobiotechnologies, Belvaux, Belgien
  • Michael Hansen, Exiqon, Danmark
  • Steve Bowra, Verzyme (UK) Ltd., Storbritannien
  • Zoltan Elek, Hungarian Academy of Sciences, Animal Ecology Research Group, Ungarn
  • Éva Vincze

The aim of the study was to understand the molecular and biochemical mechanisms underpinning the effect of nitrogen (N) on barley (Hordeum vulgare) storage protein production (hordeins) during grain filling. Using a combination of advanced biochemistry methods, we could comprehensively describe changes in transcripts (using DNA microarray and qRT-PCR), amino acids (Ultra Performance Liquid Chromatography) and protein abundance (2D-DIGE combined with MS/MS). Examining all known hordein homologues revealed markedly different temporal profiles in their expression patterns. Furthermore, different N-regimes caused significant differences in both quantity and quality of the storage proteins transcripts. Principal Component Analysis of the amino acid (AA) profiles also indicated dissimilarity in individual AA percentages, correlated to hordein content. The abundance values of proteins of interest confirmed the various hordein patterns during grain development and the differential response to environmental stimuli.

OriginalsprogEngelsk
TitelProceedings of 19th EUCARPIA General Congress : Plant Breeding for Future Generations
RedaktørerZoltan Bedo, Laszlo Lang
Antal sider4
Udgivelsesår2012
Sider176-179
ISBN (trykt)978-963-8351-39-5
StatusUdgivet - 2012
Begivenhed19th EUCARPIA General Congress: Plant Breeding for Future Generations - Budapest, Ungarn
Varighed: 21 maj 201224 maj 2012

Konference

Konference19th EUCARPIA General Congress
LandUngarn
ByBudapest
Periode21/05/201224/05/2012

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