Introduction: High-quality chest compressions and early defibrillation is essential to improve survival following in-hospital cardiac arrest. Efficient training in basic life support (BLS) for clinical staff is therefore important. This study aimed to investigate duration, training methods and retraining intervals for BLS training of clinical staff in Danish hospitals.Methods: We included all public, somatic hospitals in Denmark with a cardiac arrest team. Online questionnaires were distributed to resuscitation officers in each hospital. Questionnaires inquired information on: A) Course duration and retraining interval, and B) Training methods and setting.Results: In total, 44 hospitals replied (response rate: 96%). BLS training for clinical staff was conducted in 41 hospitals (93%). Median (Q1;Q3) course duration was 1.5 (1;2.5) hours. Retraining was conducted every year (17%), every second year (56%) or every third year (20%). In 3 (7%) hospitals retraining was never conducted. Hospitals had a median of 7 (6;10) participants per instructor and a median of 6 (2;8) participants per training manikin. In 39% of hospitals half an hour or less was spent on hands-on training (median (Q1;Q3): 1 (0.5;1.5)). Hands-on training included 30:2 and compressions only CPR by 63% while 30:2 was the only method trained by 32%. Hand-on training was not offered in 5% of hospitals. Overall, 80% included training using an AED. Chest compressions were trained in bed (63%), on the floor (46%), on a table (34%) or on a gurney (29%). Backboards were used in 2% of hospitals. Work position during chest compressions was part of training, and included adjusting the bed height (70%), removing the bed rail (59%), and/or jumping onto the bed (41%). CPR feedback devices were used by 85%. An end-of-course test for BLS skills was used in 18% of hospitals.Conclusion: There is a major difference in duration and retraining intervals for BLS training of clinical staff in Danish hospitals. Approximately half of hospitals spent less than one hour on hands-on training. Chest compressions were practiced on different surfaces using different methods to optimize work position. Backboards are very rarely used. Most hospitals did not use end-of-course tests for BLS skills.Author Disclosures: D.K. Rasmussen: None. K.G. Lauridsen: None. M. Staerk: None. T. Mygind: None. B. Loefgren: None.