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Inhibiting pri-miRNA processing with target site blockers

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Standard

Inhibiting pri-miRNA processing with target site blockers. / Louloupi, Annita; Ørom, Ulf Andersson Vang.

miRNA Biogenesis. red. / U. Ørum. Springer, 2018. s. 63-68 (Methods in Molecular Biology, Bind 1823).

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

Harvard

Louloupi, A & Ørom, UAV 2018, Inhibiting pri-miRNA processing with target site blockers. i U Ørum (red.), miRNA Biogenesis. Springer, Methods in Molecular Biology, bind 1823, s. 63-68. https://doi.org/10.1007/978-1-4939-8624-8_6

APA

Louloupi, A., & Ørom, U. A. V. (2018). Inhibiting pri-miRNA processing with target site blockers. I U. Ørum (red.), miRNA Biogenesis (s. 63-68). Springer. Methods in Molecular Biology, Bind. 1823 https://doi.org/10.1007/978-1-4939-8624-8_6

CBE

Louloupi A, Ørom UAV. 2018. Inhibiting pri-miRNA processing with target site blockers. Ørum U, red. I miRNA Biogenesis. Springer. s. 63-68. (Methods in Molecular Biology, Bind 1823). https://doi.org/10.1007/978-1-4939-8624-8_6

MLA

Louloupi, Annita og Ulf Andersson Vang Ørom "Inhibiting pri-miRNA processing with target site blockers". Ørum, U. (redaktører). miRNA Biogenesis. Springer. (Methods in Molecular Biology, Bind 1823). 2018, 63-68. https://doi.org/10.1007/978-1-4939-8624-8_6

Vancouver

Louloupi A, Ørom UAV. Inhibiting pri-miRNA processing with target site blockers. I Ørum U, red., miRNA Biogenesis. Springer. 2018. s. 63-68. (Methods in Molecular Biology, Bind 1823). https://doi.org/10.1007/978-1-4939-8624-8_6

Author

Louloupi, Annita ; Ørom, Ulf Andersson Vang. / Inhibiting pri-miRNA processing with target site blockers. miRNA Biogenesis. red. / U. Ørum. Springer, 2018. s. 63-68 (Methods in Molecular Biology, Bind 1823).

Bibtex

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title = "Inhibiting pri-miRNA processing with target site blockers",
abstract = "The pri-miRNA processing is important for the final regulatory role of miRNAs on the expression of their target transcripts. The processing variability between pri-miRNAs can determine the final miRNA abundance better than primary transcription itself. Thus studying the in vivo pri-miRNA biogenesis could give more insights into the contribution of each individual miRNA on regulation of gene expression. Interfering processing of a specific pri-miRNA has been challenging due to the nature of the current RNA interfence methods. Here, we describe step by step a method to arrest processing of specific pri-miRNAs in vivo using LNA microRNA Target Site Blockers. We explain in detail the various aspects of this approach that can easily be applied to different mammalian cell types. The nature of this protocol allows the in vivo study of pri-miRNA processing and processing kinetics in cells treated with different conditions, mutants, and/or cancer cell lines under physiological conditions.",
keywords = "BrU pulse-chase labeling, Pri-miRNA processing dynamics",
author = "Annita Louloupi and {\O}rom, {Ulf Andersson Vang}",
year = "2018",
month = "1",
doi = "10.1007/978-1-4939-8624-8_6",
language = "English",
isbn = "978-1-4939-8623-1",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "63--68",
editor = "{\O}rum, {U. }",
booktitle = "miRNA Biogenesis",

}

RIS

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T1 - Inhibiting pri-miRNA processing with target site blockers

AU - Louloupi, Annita

AU - Ørom, Ulf Andersson Vang

PY - 2018/1

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N2 - The pri-miRNA processing is important for the final regulatory role of miRNAs on the expression of their target transcripts. The processing variability between pri-miRNAs can determine the final miRNA abundance better than primary transcription itself. Thus studying the in vivo pri-miRNA biogenesis could give more insights into the contribution of each individual miRNA on regulation of gene expression. Interfering processing of a specific pri-miRNA has been challenging due to the nature of the current RNA interfence methods. Here, we describe step by step a method to arrest processing of specific pri-miRNAs in vivo using LNA microRNA Target Site Blockers. We explain in detail the various aspects of this approach that can easily be applied to different mammalian cell types. The nature of this protocol allows the in vivo study of pri-miRNA processing and processing kinetics in cells treated with different conditions, mutants, and/or cancer cell lines under physiological conditions.

AB - The pri-miRNA processing is important for the final regulatory role of miRNAs on the expression of their target transcripts. The processing variability between pri-miRNAs can determine the final miRNA abundance better than primary transcription itself. Thus studying the in vivo pri-miRNA biogenesis could give more insights into the contribution of each individual miRNA on regulation of gene expression. Interfering processing of a specific pri-miRNA has been challenging due to the nature of the current RNA interfence methods. Here, we describe step by step a method to arrest processing of specific pri-miRNAs in vivo using LNA microRNA Target Site Blockers. We explain in detail the various aspects of this approach that can easily be applied to different mammalian cell types. The nature of this protocol allows the in vivo study of pri-miRNA processing and processing kinetics in cells treated with different conditions, mutants, and/or cancer cell lines under physiological conditions.

KW - BrU pulse-chase labeling

KW - Pri-miRNA processing dynamics

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T3 - Methods in Molecular Biology

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BT - miRNA Biogenesis

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PB - Springer

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