Influence of Bacterial Profiles in Cytokine and Clinical Features of Endodontic Disease

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DOI

  • Frederico C. Martinho, State University of Campinas, São Paulo, Brazil, University of Maryland, Brasilien
  • Fabio R.M. Leite
  • Rodrigo Arruda-Vasconcelos, State University of Campinas, São Paulo, Brazil, Brasilien
  • Lidiane M. Louzada, State University of Campinas, São Paulo, Brazil, Brasilien
  • Richard P. Darveau, University of Washington, USA
  • Brenda P.F.A. Gomes, State University of Campinas, São Paulo, Brazil, Brasilien

Introduction: We verified the association between selected bacterial profiles and levels of cytokines, chemokines, and the expression of signs and symptoms of primary endodontic infection with apical periodontitis. Methods: Samples were collected from 21 root canals, and macrophages were stimulated for 24 hours. Tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-10, IL-12p70, interferon gamma, and chemokine (C-C motif) ligand 2 (CCL2) were measured using cytometric bead array. We investigated the overlapping networks between cytokines and chemokines with regression analysis. Checkerboard DNA-DNA hybridization was used to assess 40 target bacteria species. Using factor analysis, bacterial species aggregated in 2 factors. The association of bacteria species–based factors on cytokine and chemokine levels and clinical features was estimated with regression analysis. Results: A negative relationship between IL-10 (anti-inflammatory cytokine) and CCL2, TNF-α, and IFN-γ (proinflammatory cytokines) (all P <.05) was observed. CCL2 was positively correlated with TNF-α (P <.01). Thirty-eight bacteria species were detected in primary endodontic infection with apical periodontitis. The first bacteria species–based factor was associated with the size of the radiolucent area (coefficient = 15.42) and tenderness to percussion/pain on palpation (coefficient = 20.79). The second factor was associated with CCL2 levels (coefficient = 1.28). Conclusions: Different bacterial profiles can be differentially related to the expression of inflammatory proteins and the experience of clinical features.

OriginalsprogEngelsk
TidsskriftJournal of Endodontics
Vol/bind47
Nummer8
Sider (fra-til)1265-1271
Antal sider7
ISSN0099-2399
DOI
StatusUdgivet - aug. 2021

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Copyright © 2021 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

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