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Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens

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Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. / Rosenberg, Carina Agerbo; De Craeye, S.; Jongert, E.; Gargano, N.; Beghetto, E.; Del Porto, P.; Vorup-Jensen, Thomas; Petersen, Jørgen Eskild.

I: Vaccine, Bind 27, 2009, s. 2489-98.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

Rosenberg, C. A., De Craeye, S., Jongert, E., Gargano, N., Beghetto, E., Del Porto, P., ... Petersen, J. E. (2009). Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. Vaccine, 27, 2489-98.

CBE

MLA

Vancouver

Rosenberg CA, De Craeye S, Jongert E, Gargano N, Beghetto E, Del Porto P o.a. Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. Vaccine. 2009;27:2489-98.

Author

Rosenberg, Carina Agerbo ; De Craeye, S. ; Jongert, E. ; Gargano, N. ; Beghetto, E. ; Del Porto, P. ; Vorup-Jensen, Thomas ; Petersen, Jørgen Eskild. / Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. I: Vaccine. 2009 ; Bind 27. s. 2489-98.

Bibtex

@article{70cc49a01d2711de8317000ea68e967b,
title = "Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens",
abstract = "Infection with the obligate intracellular parasite Toxoplasma gondii is a significant source of parasitic infections worldwide. In adults, infections may often lead to severe retinochoroiditis. Infection of the foetus causes abortion or congenital pathology that may lead to neurological complications. Although several strategies have been suggested for making a vaccine, none is currently available. Here, we investigate the protection conferred by DNA vaccination with two constructs, pcEC2 (MIC2-MIC3-SAG1) and pcEC3 (GRA3-GRA7-M2AP), encoding chimeric proteins containing multiple antigenic sequences from T. gondii. After challenge with a T. gondii genotype II, but not a genotype III strain, a significant decrease in cerebral cyst load was found compared to the controls. The immune protection involved a cell-mediated immune response with the synthesis of the cytokines IFN-? and IL-10. In silico structure analysis and the expression profile of EC2, suggest an association between antigen stability, the degree of protein secondary structure and induction of cellular immune responses. Intracellular protein degradation is an important step in the pathway leading to presentation of antigenic peptides on Major Histocompatibility Complex molecules. We suggest that degradation of this chimeric protein may have contributed to the induction of a cellular immune response via enhanced presentation of antigenic peptides on Major Histocompatibility Complex class I molecules.",
author = "Rosenberg, {Carina Agerbo} and {De Craeye}, S. and E. Jongert and N. Gargano and E. Beghetto and {Del Porto}, P. and Thomas Vorup-Jensen and Petersen, {J{\o}rgen Eskild}",
year = "2009",
language = "English",
volume = "27",
pages = "2489--98",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens

AU - Rosenberg, Carina Agerbo

AU - De Craeye, S.

AU - Jongert, E.

AU - Gargano, N.

AU - Beghetto, E.

AU - Del Porto, P.

AU - Vorup-Jensen, Thomas

AU - Petersen, Jørgen Eskild

PY - 2009

Y1 - 2009

N2 - Infection with the obligate intracellular parasite Toxoplasma gondii is a significant source of parasitic infections worldwide. In adults, infections may often lead to severe retinochoroiditis. Infection of the foetus causes abortion or congenital pathology that may lead to neurological complications. Although several strategies have been suggested for making a vaccine, none is currently available. Here, we investigate the protection conferred by DNA vaccination with two constructs, pcEC2 (MIC2-MIC3-SAG1) and pcEC3 (GRA3-GRA7-M2AP), encoding chimeric proteins containing multiple antigenic sequences from T. gondii. After challenge with a T. gondii genotype II, but not a genotype III strain, a significant decrease in cerebral cyst load was found compared to the controls. The immune protection involved a cell-mediated immune response with the synthesis of the cytokines IFN-? and IL-10. In silico structure analysis and the expression profile of EC2, suggest an association between antigen stability, the degree of protein secondary structure and induction of cellular immune responses. Intracellular protein degradation is an important step in the pathway leading to presentation of antigenic peptides on Major Histocompatibility Complex molecules. We suggest that degradation of this chimeric protein may have contributed to the induction of a cellular immune response via enhanced presentation of antigenic peptides on Major Histocompatibility Complex class I molecules.

AB - Infection with the obligate intracellular parasite Toxoplasma gondii is a significant source of parasitic infections worldwide. In adults, infections may often lead to severe retinochoroiditis. Infection of the foetus causes abortion or congenital pathology that may lead to neurological complications. Although several strategies have been suggested for making a vaccine, none is currently available. Here, we investigate the protection conferred by DNA vaccination with two constructs, pcEC2 (MIC2-MIC3-SAG1) and pcEC3 (GRA3-GRA7-M2AP), encoding chimeric proteins containing multiple antigenic sequences from T. gondii. After challenge with a T. gondii genotype II, but not a genotype III strain, a significant decrease in cerebral cyst load was found compared to the controls. The immune protection involved a cell-mediated immune response with the synthesis of the cytokines IFN-? and IL-10. In silico structure analysis and the expression profile of EC2, suggest an association between antigen stability, the degree of protein secondary structure and induction of cellular immune responses. Intracellular protein degradation is an important step in the pathway leading to presentation of antigenic peptides on Major Histocompatibility Complex molecules. We suggest that degradation of this chimeric protein may have contributed to the induction of a cellular immune response via enhanced presentation of antigenic peptides on Major Histocompatibility Complex class I molecules.

M3 - Journal article

VL - 27

SP - 2489

EP - 2498

JO - Vaccine

JF - Vaccine

SN - 0264-410X

ER -