In situ Surface Charge Density Visualization of Self-assembled DNA Nanostructures after Ion Exchange

Steffan Moller Sonderskov, Lasse Hyldgaard Klausen, Sebastian Amland Skaanvik, Xiaojun Han, Mingdong Dong

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Abstract

The charge density of DNA is a key parameter in strand hybridization and for the interactions occurring between DNA and molecules in biological systems. Due to the intricate structure of DNA, visualization of the surface charge density of DNA nanostructures under physiological conditions was not previously possible. Here, we perform a simultaneous analysis of the topography and surface charge density of DNA nanostructures using atomic force microscopy and scanning ion conductance microscopy. The effect of in situ ion exchange using various alkali metal ions is tested with respect to the adsorption of DNA origami onto mica, and a quantitative study of surface charge density reveals ion exchange phenomena in mica as a key parameter in DNA adsorption. This is important for structure-function studies of DNA nanostructures. The research provides an efficient approach to study surface charge density of DNA origami nanostructures and other biological molecules at a single molecule level.

OriginalsprogEngelsk
TidsskriftChemPhysChem
Vol/bind21
Nummer13
Sider (fra-til)1474-1482
Antal sider9
ISSN1439-4235
DOI
StatusUdgivet - jul. 2020

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