In situ structure/function studies in wastewater treatment systems

TY - GEN

T1 - In situ structure/function studies in wastewater treatment systems

AU - Schramm, Andreas

AU - De Beer, Dirk

AU - Van Den Heuvel, Han

AU - Ottengraf, Simon

AU - Amann, Rudolf

PY - 1998/1/1

Y1 - 1998/1/1

N2 - As an example of the importance and the potential of in situ structure/function studies, nitrifying aggregates from different zones of a lab-scale fluidised bed reactor were analysed by microelectrode measurements of O2, NH4+, NO2-, and NO3- and in situ hybridisation targeting the 16S rRNA of the nitrifying bacteria. A shift from an ammonia oxidising to a nitrite oxidising community is present along the reactor. In the single aggregates an active nitrifying shell of about 100 μm could be related to the maximum abundance of nitrifiers in this zone. Interestingly, the main actors in this system are not representatives of the well-described genera Nitrosomonas and Nitrobacter but some other ammonia oxidisers from the beta subclass of Proteobacteria and a thus far unknown nitrite oxidising population.

AB - As an example of the importance and the potential of in situ structure/function studies, nitrifying aggregates from different zones of a lab-scale fluidised bed reactor were analysed by microelectrode measurements of O2, NH4+, NO2-, and NO3- and in situ hybridisation targeting the 16S rRNA of the nitrifying bacteria. A shift from an ammonia oxidising to a nitrite oxidising community is present along the reactor. In the single aggregates an active nitrifying shell of about 100 μm could be related to the maximum abundance of nitrifiers in this zone. Interestingly, the main actors in this system are not representatives of the well-described genera Nitrosomonas and Nitrobacter but some other ammonia oxidisers from the beta subclass of Proteobacteria and a thus far unknown nitrite oxidising population.

KW - Fluorescent in situ hybridisation (FISH)

KW - In situ analysis

KW - Microelectrodes

KW - Nitrification

KW - Wastewater

UR - http://www.scopus.com/inward/record.url?scp=0031832135&partnerID=8YFLogxK

U2 - 10.1016/S0273-1223(98)00140-1

DO - 10.1016/S0273-1223(98)00140-1

M3 - Conference article

AN - SCOPUS:0031832135

SN - 0273-1223

VL - 37

SP - 413

EP - 416

JO - Water Science and Technology

JF - Water Science and Technology

IS - 4-5

T2 - Proceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes

Y2 - 21 July 1997 through 23 July 1997

ER -