TY - JOUR
T1 - In situ immobilization multi-enzyme biocatalytic system on covalent organic frameworks for efficient conversion of lignocellulose to glucose
AU - Tian, Pengjiao
AU - Yang, Mingxin
AU - Muhmood, Atif
AU - Yu, Haizhong
AU - Wang, Xiqing
AU - Sun, Yonglin
PY - 2024/11
Y1 - 2024/11
N2 - Efficient enzyme immobilization is crucial for addressing the resource utilization challenges associated with lignocellulose. However, the widespread application of immobilized enzyme systems faces significant obstacles, including low enzyme activity and the limited pore structure of existing carriers. To overcome these challenges, a novel multi-enzyme biocatalytic system (multi-enzymes@COF) was developed for the in situ immobilization of cellulose and β-glucosidase on covalent organic frameworks (COFs). Results showed that multi-enzyme@COF exhibits good crystallinity and a mesoporous structure, leading to an increased enzyme loading rate of 0.6 g/g and enhanced cellulose conversion efficiency of up to 78.7 %. Additionally, multi-enzymes@COF demonstrated remarkable stability a broader pH range (4−7) and temperature range (50–70 ℃), with the actively above 70 %. Moreover, the enzymes maintained approximately 74.7 % of their activity even after seven cycles. This research presents an innovative strategy for the effective utilization of lignocellulose through enzymatic processes, promoting sustainable and efficient resource utilization.
AB - Efficient enzyme immobilization is crucial for addressing the resource utilization challenges associated with lignocellulose. However, the widespread application of immobilized enzyme systems faces significant obstacles, including low enzyme activity and the limited pore structure of existing carriers. To overcome these challenges, a novel multi-enzyme biocatalytic system (multi-enzymes@COF) was developed for the in situ immobilization of cellulose and β-glucosidase on covalent organic frameworks (COFs). Results showed that multi-enzyme@COF exhibits good crystallinity and a mesoporous structure, leading to an increased enzyme loading rate of 0.6 g/g and enhanced cellulose conversion efficiency of up to 78.7 %. Additionally, multi-enzymes@COF demonstrated remarkable stability a broader pH range (4−7) and temperature range (50–70 ℃), with the actively above 70 %. Moreover, the enzymes maintained approximately 74.7 % of their activity even after seven cycles. This research presents an innovative strategy for the effective utilization of lignocellulose through enzymatic processes, promoting sustainable and efficient resource utilization.
KW - Biocatalytic
KW - In-situ immobilization
KW - Lignocellulose
KW - Mesoporous materials
KW - Multi-enzymes
UR - http://www.scopus.com/inward/record.url?scp=85202025102&partnerID=8YFLogxK
U2 - 10.1016/j.eti.2024.103796
DO - 10.1016/j.eti.2024.103796
M3 - Journal article
AN - SCOPUS:85202025102
SN - 2352-1864
VL - 36
JO - Environmental Technology and Innovation
JF - Environmental Technology and Innovation
M1 - 103796
ER -