Improving the maturation rate of human oocytes collected ex vivo during the cryopreservation of ovarian tissue

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  • Dmitry Nikiforov, University of Copenhagen, Faculty of Health Sciences, University of Teramo
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  • Cheng Junping, University of Copenhagen, Faculty of Health Sciences, People’s Hospital of Guangxi Autonomous Region
  • ,
  • Jesus Cadenas, University of Copenhagen, Faculty of Health Sciences
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  • Vallari Shukla, Kobenhavns Universitet
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  • Robert Blanshard, Illumina UK Ltd., University of Sussex
  • ,
  • Susanne Elisabeth Pors, University of Copenhagen, Faculty of Health Sciences
  • ,
  • Stine Gry Kristensen, University of Copenhagen, Faculty of Health Sciences
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  • Kirsten Tryde Macklon, Rigshospitalet
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  • Lotte Colmorn, Rigshospitalet
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  • Erik Ernst
  • Anne Mette Bay-Bjørn
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  • Zeinab Ghezelayagh, University of Copenhagen, Faculty of Health Sciences
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  • Yu Wakimoto, University of Copenhagen, Faculty of Health Sciences, Hyogo College of Medicine
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  • Marie Louise Grøndahl, Gentofte Hospital
  • ,
  • Eva Hoffmann, Kobenhavns Universitet
  • ,
  • Claus Yding Andersen, Laboratory of Reproductive Biology, University of Copenhagen, Faculty of Health Sciences

Purpose: The aim of the present study was to improve the in vitro maturation (IVM) procedure using oocytes from surplus ovarian tissue after fertility preservation. Methods: Twenty-five patients aged 17–37 years were included in the study. Maturation was compared between oocytes collected in HEPES-buffered medium or saline, and we determined whether transport on ice prior to oocyte collection affected maturation. Two different IVM media were used that were supplemented with and without recombinant human midkine. Mature oocytes were assessed for aneuploidy using next-generation sequencing (NGS). Results: On average, 36 immature oocytes were collected from each patient (range 7–90, N = 895). Oocytes recovered from HEPES-buffered medium matured at a higher rate than oocytes recovered from saline (36% vs 26%, p < 0.01). Ovarian transportation on ice prior to the procedure negatively affected maturation compared with non-transported samples (42% vs 27%, p < 0.01). The addition of midkine improved maturation rate (34% vs 27%, p < 0.05). On average, 11 MII oocytes were obtained per patient (range 1–30). NGS of 53 MII oocytes and their first polar bodies indicated that 64% were euploid. Conclusions: The study demonstrated unexpectedly high number of immature oocytes collected from surplus ovarian tissue without any stimulation. The overall MII rate was one in three, resulting in a total number of MII oocytes that was similar to the number obtained after ovarian stimulation. If these MII oocytes prove suitable for IVF, they will provide a substantial improvement in fertility preservation for patients and advance IVM as an interesting platform for further improvements in assisted reproduction.

OriginalsprogEngelsk
TidsskriftJournal of Assisted Reproduction and Genetics
Vol/bind37
Nummer4
Sider (fra-til)891-904
Antal sider14
ISSN1058-0468
DOI
StatusUdgivet - 2020

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