Aarhus University Seal / Aarhus Universitets segl

Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp. / Bek, Anita S; Sauer, Jørgen; Thygesen, Mikkel B; Duus, Jens Ø; Petersen, Bent O; Thirup, Søren; James, Euan; Jensen, Knud J; Stougaard, Jens; Radutoiu, Simona.

I: Molecular Plant - Microbe Interactions, Bind 23, Nr. 1, 2010, s. 58-66.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bek, Anita S ; Sauer, Jørgen ; Thygesen, Mikkel B ; Duus, Jens Ø ; Petersen, Bent O ; Thirup, Søren ; James, Euan ; Jensen, Knud J ; Stougaard, Jens ; Radutoiu, Simona. / Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp. I: Molecular Plant - Microbe Interactions. 2010 ; Bind 23, Nr. 1. s. 58-66.

Bibtex

@article{bb9566b0fa0311de9c17000ea68e967b,
title = "Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp",
abstract = "Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.",
author = "Bek, {Anita S} and J{\o}rgen Sauer and Thygesen, {Mikkel B} and Duus, {Jens {\O}} and Petersen, {Bent O} and S{\o}ren Thirup and Euan James and Jensen, {Knud J} and Jens Stougaard and Simona Radutoiu",
year = "2010",
doi = "10.1094/MPMI-23-1-0058",
language = "English",
volume = "23",
pages = "58--66",
journal = "Molecular Plant - Microbe Interactions",
issn = "0894-0282",
publisher = "AMER PHYTOPATHOLOGICAL SOC",
number = "1",

}

RIS

TY - JOUR

T1 - Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp

AU - Bek, Anita S

AU - Sauer, Jørgen

AU - Thygesen, Mikkel B

AU - Duus, Jens Ø

AU - Petersen, Bent O

AU - Thirup, Søren

AU - James, Euan

AU - Jensen, Knud J

AU - Stougaard, Jens

AU - Radutoiu, Simona

PY - 2010

Y1 - 2010

N2 - Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.

AB - Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.

U2 - 10.1094/MPMI-23-1-0058

DO - 10.1094/MPMI-23-1-0058

M3 - Journal article

C2 - 19958139

VL - 23

SP - 58

EP - 66

JO - Molecular Plant - Microbe Interactions

JF - Molecular Plant - Microbe Interactions

SN - 0894-0282

IS - 1

ER -