Impact of fermentation and addition of non-starch polysaccharide-degrading enzymes on microbial population and on digestibility of dried distillers grains with solubles in pigs

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Impact of fermentation and addition of non-starch polysaccharide-degrading enzymes on microbial population and on digestibility of dried distillers grains with solubles in pigs. / Venås Jakobsen, Grethe; Jensen, Bent Borg; Knudsen, Knud Erik Bach; Canibe, Nuria.

I: Livestock Science, Bind 178, 08.2015, s. 216-227.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{8fcb83558c2446c4b6459f99d187dc3a,
title = "Impact of fermentation and addition of non-starch polysaccharide-degrading enzymes on microbial population and on digestibility of dried distillers grains with solubles in pigs",
abstract = "Fluctuating prices on feedstock has led to a growing interest in alternative feed ingredients. Co-products from the biofuel industry are hence interesting to include in pig feeds, primarily due to the high protein content. Low nutritional value due to a high content of dietary fibre, however, limits the use of these ingredients. The current study aimed to increase the digestibility of dried distillers grains with solubles (DDGS), based on 80{\%} wheat and 20{\%} barley, by fermentation and enzyme addition. Four experimental diets were prepared based on 60{\%} DDGS (fermented or not, without or with the addition of carbohydrases) and 40{\%} of a basal diet composed mainly of maize starch: (1) a nonfermented liquid feed treatment (n-FLF); (2) a fermented liquid feed treatment (FLF); (3) the FLF supplemented with a mixture of xylanase and β-glucanase (XylGlu); and (4) the FLF supplemented with a mixture of cellulase and xylanase (CelXyl). Microbial population during fermentation of the treatments was determined and apparent ileal and total tract digestibility were measured on eight barrows surgically fitted with a simple T-shaped cannula at the distal ileum and fed the four treatments according to a double-Latin square design. Microbial activity of the three fermented DDGS treatments was relatively low with lactic acid bacteria counts between 8.8 and 8.9 log cfu/g and lactic acid concentrations between 60.2 and 70.5 mmol/kg. The addition of CelXyl to DDGS resulted in a significant decrease in the amount of non-starch polysaccharides (NSP) compared to the fermented DDGS (from 341.8 g/kg DM to 312.9 g/kg DM, P<0.001). Furthermore, addition of CelXyl to DDGS showed a reduction in the level of putrescine (46.5 mg/kg DM in the DDGS versus 3.7 mg/kg DM in the DDGS added CelXyl). Fermenting DDGS prior to feeding led to significantly increased apparent ileal digestibility (AID) of NSP (P=0.02), and apparent total tract digestibility (ATTD) of DM (P=0.01), CP (P=0.003), and P (P=0.04), along with tendency to increased ATTD of NSP (P=0.1). Addition of XylGlu and CelXyl resulted in further increases of both AID and ATTD of NSP compared to n-FLF (P≤0.03); with the greatest effect on the CelXyl treatment. In conclusion, the digestibility of DDGS in pigs was increased by fermentation. Addition of carbohydrases during fermentation can be a way of further increasing the NSP digestibility of DDGS.",
keywords = "Dried distillers grains with solubles, Pigs , Digestibility, Carbohydrases, Fermented liquid feed",
author = "{Ven{\aa}s Jakobsen}, Grethe and Jensen, {Bent Borg} and Knudsen, {Knud Erik Bach} and Nuria Canibe",
year = "2015",
month = "8",
doi = "10.1016/j.livsci.2015.05.028",
language = "English",
volume = "178",
pages = "216--227",
journal = "Livestock Science",
issn = "1871-1413",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Impact of fermentation and addition of non-starch polysaccharide-degrading enzymes on microbial population and on digestibility of dried distillers grains with solubles in pigs

AU - Venås Jakobsen, Grethe

AU - Jensen, Bent Borg

AU - Knudsen, Knud Erik Bach

AU - Canibe, Nuria

PY - 2015/8

Y1 - 2015/8

N2 - Fluctuating prices on feedstock has led to a growing interest in alternative feed ingredients. Co-products from the biofuel industry are hence interesting to include in pig feeds, primarily due to the high protein content. Low nutritional value due to a high content of dietary fibre, however, limits the use of these ingredients. The current study aimed to increase the digestibility of dried distillers grains with solubles (DDGS), based on 80% wheat and 20% barley, by fermentation and enzyme addition. Four experimental diets were prepared based on 60% DDGS (fermented or not, without or with the addition of carbohydrases) and 40% of a basal diet composed mainly of maize starch: (1) a nonfermented liquid feed treatment (n-FLF); (2) a fermented liquid feed treatment (FLF); (3) the FLF supplemented with a mixture of xylanase and β-glucanase (XylGlu); and (4) the FLF supplemented with a mixture of cellulase and xylanase (CelXyl). Microbial population during fermentation of the treatments was determined and apparent ileal and total tract digestibility were measured on eight barrows surgically fitted with a simple T-shaped cannula at the distal ileum and fed the four treatments according to a double-Latin square design. Microbial activity of the three fermented DDGS treatments was relatively low with lactic acid bacteria counts between 8.8 and 8.9 log cfu/g and lactic acid concentrations between 60.2 and 70.5 mmol/kg. The addition of CelXyl to DDGS resulted in a significant decrease in the amount of non-starch polysaccharides (NSP) compared to the fermented DDGS (from 341.8 g/kg DM to 312.9 g/kg DM, P<0.001). Furthermore, addition of CelXyl to DDGS showed a reduction in the level of putrescine (46.5 mg/kg DM in the DDGS versus 3.7 mg/kg DM in the DDGS added CelXyl). Fermenting DDGS prior to feeding led to significantly increased apparent ileal digestibility (AID) of NSP (P=0.02), and apparent total tract digestibility (ATTD) of DM (P=0.01), CP (P=0.003), and P (P=0.04), along with tendency to increased ATTD of NSP (P=0.1). Addition of XylGlu and CelXyl resulted in further increases of both AID and ATTD of NSP compared to n-FLF (P≤0.03); with the greatest effect on the CelXyl treatment. In conclusion, the digestibility of DDGS in pigs was increased by fermentation. Addition of carbohydrases during fermentation can be a way of further increasing the NSP digestibility of DDGS.

AB - Fluctuating prices on feedstock has led to a growing interest in alternative feed ingredients. Co-products from the biofuel industry are hence interesting to include in pig feeds, primarily due to the high protein content. Low nutritional value due to a high content of dietary fibre, however, limits the use of these ingredients. The current study aimed to increase the digestibility of dried distillers grains with solubles (DDGS), based on 80% wheat and 20% barley, by fermentation and enzyme addition. Four experimental diets were prepared based on 60% DDGS (fermented or not, without or with the addition of carbohydrases) and 40% of a basal diet composed mainly of maize starch: (1) a nonfermented liquid feed treatment (n-FLF); (2) a fermented liquid feed treatment (FLF); (3) the FLF supplemented with a mixture of xylanase and β-glucanase (XylGlu); and (4) the FLF supplemented with a mixture of cellulase and xylanase (CelXyl). Microbial population during fermentation of the treatments was determined and apparent ileal and total tract digestibility were measured on eight barrows surgically fitted with a simple T-shaped cannula at the distal ileum and fed the four treatments according to a double-Latin square design. Microbial activity of the three fermented DDGS treatments was relatively low with lactic acid bacteria counts between 8.8 and 8.9 log cfu/g and lactic acid concentrations between 60.2 and 70.5 mmol/kg. The addition of CelXyl to DDGS resulted in a significant decrease in the amount of non-starch polysaccharides (NSP) compared to the fermented DDGS (from 341.8 g/kg DM to 312.9 g/kg DM, P<0.001). Furthermore, addition of CelXyl to DDGS showed a reduction in the level of putrescine (46.5 mg/kg DM in the DDGS versus 3.7 mg/kg DM in the DDGS added CelXyl). Fermenting DDGS prior to feeding led to significantly increased apparent ileal digestibility (AID) of NSP (P=0.02), and apparent total tract digestibility (ATTD) of DM (P=0.01), CP (P=0.003), and P (P=0.04), along with tendency to increased ATTD of NSP (P=0.1). Addition of XylGlu and CelXyl resulted in further increases of both AID and ATTD of NSP compared to n-FLF (P≤0.03); with the greatest effect on the CelXyl treatment. In conclusion, the digestibility of DDGS in pigs was increased by fermentation. Addition of carbohydrases during fermentation can be a way of further increasing the NSP digestibility of DDGS.

KW - Dried distillers grains with solubles

KW - Pigs

KW - Digestibility

KW - Carbohydrases

KW - Fermented liquid feed

U2 - 10.1016/j.livsci.2015.05.028

DO - 10.1016/j.livsci.2015.05.028

M3 - Journal article

VL - 178

SP - 216

EP - 227

JO - Livestock Science

JF - Livestock Science

SN - 1871-1413

ER -