TY - JOUR
T1 - In situ biomethanation
T2 - Inoculum origin influences acetate consumption rate during hydrogen addition
AU - Dos Reis Vechi, Nathalia
AU - Agnessens, Laura Mia
AU - Feilberg, Anders
AU - Ottosen, Lars Ditlev Mørck
AU - Kofoed, Michael Vedel Wegener
PY - 2021/6
Y1 - 2021/6
N2 - Biological conversion of carbon dioxide (CO
2) to methane (CH
4) through the exogenous addition of hydrogen (H
2) represent a promising biotechnology for biogas upgrading and energy conversion through the process of H
2-mediated biomethanation. Acetate accumulation has however been observed during H
2 addition, which may deteriorate process stability. Using
13C-isotope techniques, we found that acetate consumption declined by 84% upon H
2 addition in digestate from lab-scale digesters treating agricultural waste products (AD-Manure) but did not decline in digestate based on wastewater treatment sludge (AD-WWT). Dominance of different acetoclastic methanogens, along with the fact that the H
2-sensitive process of syntrophic acetate oxidation (SAO) was responsible for converting 27% of the acetate in AD-Manure and only 4% in AD-WWT, could explain the different response of AD-Manure and AD-WWT. Our results improve the understanding of acetate consumption rates during H
2 addition in reactors treating different organic feedstock, and how these are suited for H
2-mediated biomethanation.
AB - Biological conversion of carbon dioxide (CO
2) to methane (CH
4) through the exogenous addition of hydrogen (H
2) represent a promising biotechnology for biogas upgrading and energy conversion through the process of H
2-mediated biomethanation. Acetate accumulation has however been observed during H
2 addition, which may deteriorate process stability. Using
13C-isotope techniques, we found that acetate consumption declined by 84% upon H
2 addition in digestate from lab-scale digesters treating agricultural waste products (AD-Manure) but did not decline in digestate based on wastewater treatment sludge (AD-WWT). Dominance of different acetoclastic methanogens, along with the fact that the H
2-sensitive process of syntrophic acetate oxidation (SAO) was responsible for converting 27% of the acetate in AD-Manure and only 4% in AD-WWT, could explain the different response of AD-Manure and AD-WWT. Our results improve the understanding of acetate consumption rates during H
2 addition in reactors treating different organic feedstock, and how these are suited for H
2-mediated biomethanation.
KW - Acetate
KW - Acetoclastic methanogesis
KW - Biomethanation
KW - Homoacetogenesis
KW - Hydrogen
KW - Syntrophic acetate oxidation
UR - http://www.scopus.com/inward/record.url?scp=85102069262&partnerID=8YFLogxK
U2 - 10.1016/j.biteb.2021.100656
DO - 10.1016/j.biteb.2021.100656
M3 - Journal article
SN - 2589-014X
VL - 14
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
M1 - 100656
ER -