Aarhus University Seal / Aarhus Universitets segl

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. / Vitre, Benjamin; Taulet, Nicolas; Guesdon, Audrey; Douanier, Audrey; Dosdane, Aurelie; Cisneros, Melanie; Maurin, Justine; Hettinger, Sabrina; Anguille, Christelle; Taschner, Michael; Lorentzen, Esben; Delaval, Benedicte.

I: EMBO Reports, Bind 21, Nr. 6, e49234, 06.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Vitre, B, Taulet, N, Guesdon, A, Douanier, A, Dosdane, A, Cisneros, M, Maurin, J, Hettinger, S, Anguille, C, Taschner, M, Lorentzen, E & Delaval, B 2020, 'IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis', EMBO Reports, bind 21, nr. 6, e49234. https://doi.org/10.15252/embr.201949234

APA

Vitre, B., Taulet, N., Guesdon, A., Douanier, A., Dosdane, A., Cisneros, M., Maurin, J., Hettinger, S., Anguille, C., Taschner, M., Lorentzen, E., & Delaval, B. (2020). IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. EMBO Reports, 21(6), [e49234]. https://doi.org/10.15252/embr.201949234

CBE

Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M, Maurin J, Hettinger S, Anguille C, Taschner M, Lorentzen E, Delaval B. 2020. IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. EMBO Reports. 21(6):Article e49234. https://doi.org/10.15252/embr.201949234

MLA

Vancouver

Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M o.a. IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. EMBO Reports. 2020 jun;21(6). e49234. https://doi.org/10.15252/embr.201949234

Author

Vitre, Benjamin ; Taulet, Nicolas ; Guesdon, Audrey ; Douanier, Audrey ; Dosdane, Aurelie ; Cisneros, Melanie ; Maurin, Justine ; Hettinger, Sabrina ; Anguille, Christelle ; Taschner, Michael ; Lorentzen, Esben ; Delaval, Benedicte. / IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. I: EMBO Reports. 2020 ; Bind 21, Nr. 6.

Bibtex

@article{91f3101334c24781b1d9dffc0057345b,
title = "IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis",
abstract = "Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes.",
keywords = "cancer, centrosome clustering, HSET/KifC1, IFT proteins, mitosis",
author = "Benjamin Vitre and Nicolas Taulet and Audrey Guesdon and Audrey Douanier and Aurelie Dosdane and Melanie Cisneros and Justine Maurin and Sabrina Hettinger and Christelle Anguille and Michael Taschner and Esben Lorentzen and Benedicte Delaval",
year = "2020",
month = jun,
doi = "10.15252/embr.201949234",
language = "English",
volume = "21",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "6",

}

RIS

TY - JOUR

T1 - IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis

AU - Vitre, Benjamin

AU - Taulet, Nicolas

AU - Guesdon, Audrey

AU - Douanier, Audrey

AU - Dosdane, Aurelie

AU - Cisneros, Melanie

AU - Maurin, Justine

AU - Hettinger, Sabrina

AU - Anguille, Christelle

AU - Taschner, Michael

AU - Lorentzen, Esben

AU - Delaval, Benedicte

PY - 2020/6

Y1 - 2020/6

N2 - Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes.

AB - Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes.

KW - cancer

KW - centrosome clustering

KW - HSET/KifC1

KW - IFT proteins

KW - mitosis

UR - http://www.scopus.com/inward/record.url?scp=85083270038&partnerID=8YFLogxK

U2 - 10.15252/embr.201949234

DO - 10.15252/embr.201949234

M3 - Journal article

C2 - 32270908

AN - SCOPUS:85083270038

VL - 21

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 6

M1 - e49234

ER -