Identification of on- and off-pathway oligomers in amyloid fibril formation

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  • Alexander J. Dear, University of Cambridge, Lund University
  • ,
  • Georg Meisl, University of Cambridge
  • ,
  • Andela Saric, University College London
  • ,
  • Thomas C. T. Michaels, University of Cambridge, Harvard University
  • ,
  • Magnus Kjaergaard
  • Sara Linse, Lund University
  • ,
  • Tuomas P. J. Knowles, University of Cambridge

The misfolding and aberrant aggregation of proteins into fibrillar structures is a key factor in some of the most prevalent human diseases, including diabetes and dementia. Low molecular weight oligomers are thought to be a central factor in the pathology of these diseases, as well as critical intermediates in the fibril formation process, and as such have received much recent attention. Moreover, on-pathway oligomeric intermediates are potential targets for therapeutic strategies aimed at interrupting the fibril formation process. However, a consistent framework for distinguishing on-pathway from off-pathway oligomers has hitherto been lacking and, in particular, no consensus definition of on- and off-pathway oligomers is available. In this paper, we argue that a non-binary definition of oligomers' contribution to fibril-forming pathways may be more informative and we suggest a quantitative framework, in which each oligomeric species is assigned a value between 0 and 1 describing its relative contribution to the formation of fibrils. First, we clarify the distinction between oligomers and fibrils, and then we use the formalism of reaction networks to develop a general definition for on-pathway oligomers, that yields meaningful classifications in the context of amyloid formation. By applying these concepts to Monte Carlo simulations of a minimal aggregating system, and by revisiting several previous studies of amyloid oligomers in light of our new framework, we demonstrate how to perform these classifications in practice. For each oligomeric species we obtain the degree to which it is on-pathway, highlighting the most effective pharmaceutical targets for the inhibition of amyloid fibril formation.

OriginalsprogEngelsk
TidsskriftChemical Science
Vol/bind11
Nummer24
Sider (fra-til)6236-6247
Antal sider12
ISSN2041-6520
DOI
StatusUdgivet - jun. 2020

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