Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Toke Peter Kroager
  • ,
  • Lone Vendel Nielsen, Danmark
  • Steffen Bak, The Protein Research Group at the Department of Biochemistry and Molecular Biology, University of Southern Denmark, Danmark
  • Clifford Young, The Protein Research Group at the Department of Biochemistry and Molecular Biology, University of Southern Denmark, Danmark
  • Carla Ferreri, I.S.O.F. — BioFreeRadicals, Consiglio Nazionale delle Ricerche, Italien
  • Ole Nørregaard Jensen, Institut for Biokemi og Molekylær Biologi, Danmark
  • Peter Højrup, Institut for Biokemi og Molekylær Biologi, Danmark
  • Vladimiros Thoma, I.S.O.F. — BioFreeRadicals, Consiglio Nazionale delle Ricerche, Italien
  • Ida B Thøgersen
  • Jan J Enghild
Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value
OriginalsprogEngelsk
TidsskriftJournal of Proteomics
Vol/bind75
Nummer9
Sider (fra-til)2685-2696
Antal sider12
ISSN1874-3919
DOI
StatusUdgivet - 17 maj 2012

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