Hyperpolarized 13c magnetic resonance spectroscopy and hypoxic ischemic encephalopathy – proof of concept in a piglet model

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Background and aims:
Biomarkers of cerebral damage are key for prognostication in newborn with hypoxic ischemic encephalopathy (HIE). Hyperpolarized 13C magnetic resonance spectroscopy (MRS) is a novel method to quantify metabolism in vivo with ultra-high resolution. In this proof-of-concept study, we aimed to investigate whether hyperpolarized 13C MRS can be applied in a piglet model of HIE and used to detect early changes in metabolite profile after a standardized hypoxic ischemic (HI) insult.

Methods:
Six piglets were anesthetised and subjected to a standardised HI insult. Imaging was performed at baseline and two hours after the HI insult on a 3 T MR scanner. For 13C studies, 127 mg [1-13C]pyruvic acid was hyperpolarized in the SpinLab polarizer. Following intra venous injection (6 ml), rapid interleaved spectroscopic images were acquired, using a spectral-spatial spiral imaging sequence. Metabolism was quantified as area under the curve and determined for pyruvate, alanine, lactate, and bicarbonate.

Results:
Hyperpolarized 13C MRS was successfully performed in all animals. HI resulted in a reduction in bicarbonate metabolism (mean (SD); 116.5 (25.7) vs. 37.6 (5.9), p = 0.02). There was no change in lactate (576.6 (103.9) vs. 547.2 (100.3), p = 0.50), alanine (74.4 (12.0) vs. 68.6 (31.3), p = 0.84), or pyruvate (391.6 (69.6) vs. 263.9 (80.6), p = 0.06) at two hours.

Conclusion:
For the first time ever, we show that hyperpolarized 13C MRS can be used in the piglet and applied to evaluate early changes in cerebral metabolism after a HI insult.
OriginalsprogEngelsk
Udgivelsesår2020
StatusUdgivet - 2020
Begivenhed8th Congress of the European Academy of Paediatric Societies -
Varighed: 16 okt. 202019 okt. 2020

Konference

Konference8th Congress of the European Academy of Paediatric Societies
Periode16/10/202019/10/2020

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