Hyperphosphorylation of cereal starch

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Standard

Hyperphosphorylation of cereal starch. / Carciofi, Massimiliano; Shaik, Shahnoor Sultana; Jensen, Susanne Langgård; Blennow, Per Gunnar Andreas; Svensson, Jan Tommy; Vincze, Éva; Hebelstrup, Kim.

I: Journal of Cereal Science, Bind 54, Nr. 3, 11.2011, s. 339-346.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Carciofi, M, Shaik, SS, Jensen, SL, Blennow, PGA, Svensson, JT, Vincze, É & Hebelstrup, K 2011, 'Hyperphosphorylation of cereal starch' Journal of Cereal Science, bind 54, nr. 3, s. 339-346. https://doi.org/10.1016/j.jcs.2011.06.013

APA

Carciofi, M., Shaik, S. S., Jensen, S. L., Blennow, P. G. A., Svensson, J. T., Vincze, É., & Hebelstrup, K. (2011). Hyperphosphorylation of cereal starch. Journal of Cereal Science, 54(3), 339-346. https://doi.org/10.1016/j.jcs.2011.06.013

CBE

Carciofi M, Shaik SS, Jensen SL, Blennow PGA, Svensson JT, Vincze É, Hebelstrup K. 2011. Hyperphosphorylation of cereal starch. Journal of Cereal Science. 54(3):339-346. https://doi.org/10.1016/j.jcs.2011.06.013

MLA

Carciofi, Massimiliano o.a.. "Hyperphosphorylation of cereal starch". Journal of Cereal Science. 2011, 54(3). 339-346. https://doi.org/10.1016/j.jcs.2011.06.013

Vancouver

Carciofi M, Shaik SS, Jensen SL, Blennow PGA, Svensson JT, Vincze É o.a. Hyperphosphorylation of cereal starch. Journal of Cereal Science. 2011 nov;54(3):339-346. https://doi.org/10.1016/j.jcs.2011.06.013

Author

Carciofi, Massimiliano ; Shaik, Shahnoor Sultana ; Jensen, Susanne Langgård ; Blennow, Per Gunnar Andreas ; Svensson, Jan Tommy ; Vincze, Éva ; Hebelstrup, Kim. / Hyperphosphorylation of cereal starch. I: Journal of Cereal Science. 2011 ; Bind 54, Nr. 3. s. 339-346.

Bibtex

@article{46e0fca4da234e7c894ca13fb9750c4e,
title = "Hyperphosphorylation of cereal starch",
abstract = "Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67) nmol/mg) compared to control lines (0.8 (±0.05) nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.",
keywords = "Starch, Phosphorylation, Glucan Water Dikinase (GWD), Transgenic barley",
author = "Massimiliano Carciofi and Shaik, {Shahnoor Sultana} and Jensen, {Susanne Langg{\aa}rd} and Blennow, {Per Gunnar Andreas} and Svensson, {Jan Tommy} and {\'E}va Vincze and Kim Hebelstrup",
year = "2011",
month = "11",
doi = "10.1016/j.jcs.2011.06.013",
language = "English",
volume = "54",
pages = "339--346",
journal = "Journal of Cereal Science",
issn = "0733-5210",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - Hyperphosphorylation of cereal starch

AU - Carciofi, Massimiliano

AU - Shaik, Shahnoor Sultana

AU - Jensen, Susanne Langgård

AU - Blennow, Per Gunnar Andreas

AU - Svensson, Jan Tommy

AU - Vincze, Éva

AU - Hebelstrup, Kim

PY - 2011/11

Y1 - 2011/11

N2 - Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67) nmol/mg) compared to control lines (0.8 (±0.05) nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.

AB - Plant starch is naturally phosphorylated at a fraction of the C6 and the C3 hydroxyl groups during its biosynthesis in plastids. Starch phosphate esters are important in starch metabolism and they also generate specific industrial functionality. Cereal grains starch contains little starch bound phosphate compared with potato tuber starch and in order to investigate the effect of increased endosperm starch phosphate, the potato starch phosphorylating enzyme glucan water dikinase (StGWD) was overexpressed specifically in the developing barley endosperm. StGWD overexpressors showed wild-type phenotype. Transgenic cereal grains synthesized starch with higher starch bound phosphate content (7.5 (±0.67) nmol/mg) compared to control lines (0.8 (±0.05) nmol/mg) with starch granules showing altered morphology and lower melting enthalpy. Our data indicate specific action of GWD during starch biosynthesis and demonstrates the possibility for in planta production of highly phosphorylated cereal starch.

KW - Starch

KW - Phosphorylation

KW - Glucan Water Dikinase (GWD)

KW - Transgenic barley

U2 - 10.1016/j.jcs.2011.06.013

DO - 10.1016/j.jcs.2011.06.013

M3 - Journal article

VL - 54

SP - 339

EP - 346

JO - Journal of Cereal Science

JF - Journal of Cereal Science

SN - 0733-5210

IS - 3

ER -