Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators

Yan Zhang; Noga Gal; Fabian Itel; Isabella N. Westensee; Edit Brodszkij; Daniel Mayer; Steffen Stenger; Miquel Castellote-Borrell; Thomas Boesen; Seyed R. Tabaei; Fredrik Hook; Brigitte Stadler

doi:10.1039/c9nr02584g

Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators

Yan Zhang, Noga Gal, Fabian Itel, Isabella N. Westensee, Edit Brodszkij, Daniel Mayer, Steffen Stenger, Miquel Castellote-Borrell, Thomas Boesen, Seyed R. Tabaei, Fredrik Hook, Brigitte Stadler^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

20 Citationer (Scopus)

Abstract

Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NR _GOx) or β-galactosidase (NR _β-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NR _GOx and NR _β-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NR _GOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR _β-Gal are able to intracellularly convert β-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry.

Originalsprog	Engelsk
Tidsskrift	Nanoscale
Vol/bind	11
Nummer	24
Sider (fra-til)	11530-11541
Antal sider	12
ISSN	2040-3364
DOI	https://doi.org/10.1039/c9nr02584g
Status	Udgivet - jun. 2019

Adgang til dokumentet

10.1039/c9nr02584g

Citationsformater

@article{02e54b11ae8049c8b8d37e7fc7f84fb8,

title = "Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators",

abstract = "Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NR GOx) or β-galactosidase (NR β-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NR GOx and NR β-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NR GOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR β-Gal are able to intracellularly convert β-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry. ",

keywords = "NANOREACTORS, DELIVERY, ENZYMES, NANOPARTICLES, ORGANELLES, RELEASE",

author = "Yan Zhang and Noga Gal and Fabian Itel and Westensee, {Isabella N.} and Edit Brodszkij and Daniel Mayer and Steffen Stenger and Miquel Castellote-Borrell and Thomas Boesen and Tabaei, {Seyed R.} and Fredrik Hook and Brigitte Stadler",

year = "2019",

month = jun,

doi = "10.1039/c9nr02584g",

language = "English",

volume = "11",

pages = "11530--11541",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

number = "24",

}

TY - JOUR

T1 - Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators

AU - Zhang, Yan

AU - Gal, Noga

AU - Itel, Fabian

AU - Westensee, Isabella N.

AU - Brodszkij, Edit

AU - Mayer, Daniel

AU - Stenger, Steffen

AU - Castellote-Borrell, Miquel

AU - Boesen, Thomas

AU - Tabaei, Seyed R.

AU - Hook, Fredrik

AU - Stadler, Brigitte

PY - 2019/6

Y1 - 2019/6

N2 - Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NR GOx) or β-galactosidase (NR β-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NR GOx and NR β-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NR GOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR β-Gal are able to intracellularly convert β-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry.

AB - Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NR GOx) or β-galactosidase (NR β-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NR GOx and NR β-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NR GOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR β-Gal are able to intracellularly convert β-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry.

KW - NANOREACTORS

KW - DELIVERY

KW - ENZYMES

KW - NANOPARTICLES

KW - ORGANELLES

KW - RELEASE

U2 - 10.1039/c9nr02584g

DO - 10.1039/c9nr02584g

M3 - Journal article

C2 - 31150038

SN - 2040-3364

VL - 11

SP - 11530

EP - 11541

JO - Nanoscale

JF - Nanoscale

IS - 24

ER -

Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater