Human skin keratins are the major proteins in exhaled breath condensate

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Human skin keratins are the major proteins in exhaled breath condensate. / Hoffmann, H J; Tabaksblat, L M; Enghild, J J; Dahl, R.

I: European Respiratory Journal, Bind 31, Nr. 2, 2008, s. 380-4.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Hoffmann, H J ; Tabaksblat, L M ; Enghild, J J ; Dahl, R. / Human skin keratins are the major proteins in exhaled breath condensate. I: European Respiratory Journal. 2008 ; Bind 31, Nr. 2. s. 380-4.

Bibtex

@article{80457f80a36b11dea554000ea68e967b,
title = "Human skin keratins are the major proteins in exhaled breath condensate",
abstract = "Exhaled breath condensate (EBC) may be an attractive noninvasive alternative to bronchoscopy, bronchoalveolar lavage and induced sputum for diagnosis and monitoring of pulmonary disease. The aim of the present study was to identify proteins in EBC by mass spectrometry. Protein in EBC was characterised by gel electrophoresis of freeze-dried EBC samples, and individual proteins were identified by mass spectrometry. Saliva, ambient air condensate (AAC) and EBC were collected from normal human volunteers with or without a filter to remove particles from air. In some instances, EBC was condensed by breathing compressed air. Samples were freeze-dried and analysed by SDS-PAGE and peptide mass fingerprinting. Three major bands were seen in EBC and AAC, and were identified by peptide mass fingerprinting. The probability-based Mowse score was significant only for cytokeratin (CK) 1, CK2 and CK10. In the catalogue of human cytokeratins, CK1, CK2, CK9 and CK10 are described in keratinising epidermis. Saliva did not contain keratin and compressed air EBC contained markedly less keratin. Filtration of inspired air did not remove contaminating keratin. In conclusion, skin keratin in exhaled breath condensate derives from ambient air and not from the respiratory tract.",
keywords = "Adult, Breath Tests, Exhalation, Female, Humans, Keratins, Male, Mass Spectrometry, Middle Aged, Peptide Mapping, Reference Values, Sampling Studies, Sensitivity and Specificity, Skin",
author = "Hoffmann, {H J} and Tabaksblat, {L M} and Enghild, {J J} and R Dahl",
year = "2008",
doi = "10.1183/09031936.00059707",
language = "English",
volume = "31",
pages = "380--4",
journal = "European Respiratory Journal",
issn = "0903-1936",
publisher = "European Respiratory Society",
number = "2",

}

RIS

TY - JOUR

T1 - Human skin keratins are the major proteins in exhaled breath condensate

AU - Hoffmann, H J

AU - Tabaksblat, L M

AU - Enghild, J J

AU - Dahl, R

PY - 2008

Y1 - 2008

N2 - Exhaled breath condensate (EBC) may be an attractive noninvasive alternative to bronchoscopy, bronchoalveolar lavage and induced sputum for diagnosis and monitoring of pulmonary disease. The aim of the present study was to identify proteins in EBC by mass spectrometry. Protein in EBC was characterised by gel electrophoresis of freeze-dried EBC samples, and individual proteins were identified by mass spectrometry. Saliva, ambient air condensate (AAC) and EBC were collected from normal human volunteers with or without a filter to remove particles from air. In some instances, EBC was condensed by breathing compressed air. Samples were freeze-dried and analysed by SDS-PAGE and peptide mass fingerprinting. Three major bands were seen in EBC and AAC, and were identified by peptide mass fingerprinting. The probability-based Mowse score was significant only for cytokeratin (CK) 1, CK2 and CK10. In the catalogue of human cytokeratins, CK1, CK2, CK9 and CK10 are described in keratinising epidermis. Saliva did not contain keratin and compressed air EBC contained markedly less keratin. Filtration of inspired air did not remove contaminating keratin. In conclusion, skin keratin in exhaled breath condensate derives from ambient air and not from the respiratory tract.

AB - Exhaled breath condensate (EBC) may be an attractive noninvasive alternative to bronchoscopy, bronchoalveolar lavage and induced sputum for diagnosis and monitoring of pulmonary disease. The aim of the present study was to identify proteins in EBC by mass spectrometry. Protein in EBC was characterised by gel electrophoresis of freeze-dried EBC samples, and individual proteins were identified by mass spectrometry. Saliva, ambient air condensate (AAC) and EBC were collected from normal human volunteers with or without a filter to remove particles from air. In some instances, EBC was condensed by breathing compressed air. Samples were freeze-dried and analysed by SDS-PAGE and peptide mass fingerprinting. Three major bands were seen in EBC and AAC, and were identified by peptide mass fingerprinting. The probability-based Mowse score was significant only for cytokeratin (CK) 1, CK2 and CK10. In the catalogue of human cytokeratins, CK1, CK2, CK9 and CK10 are described in keratinising epidermis. Saliva did not contain keratin and compressed air EBC contained markedly less keratin. Filtration of inspired air did not remove contaminating keratin. In conclusion, skin keratin in exhaled breath condensate derives from ambient air and not from the respiratory tract.

KW - Adult

KW - Breath Tests

KW - Exhalation

KW - Female

KW - Humans

KW - Keratins

KW - Male

KW - Mass Spectrometry

KW - Middle Aged

KW - Peptide Mapping

KW - Reference Values

KW - Sampling Studies

KW - Sensitivity and Specificity

KW - Skin

U2 - 10.1183/09031936.00059707

DO - 10.1183/09031936.00059707

M3 - Journal article

C2 - 18238948

VL - 31

SP - 380

EP - 384

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - 2

ER -