Host microbiota dictates the proinflammatory impact of LPS in the murine liver

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Host microbiota dictates the proinflammatory impact of LPS in the murine liver. / Suriguga, Su; Luangmonkong, Theerut; Mutsaers, Henricus A.M.; Groothuis, Geny M.M.; Olinga, Peter.

I: Toxicology in Vitro, Bind 67, 104920, 09.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Suriguga, S, Luangmonkong, T, Mutsaers, HAM, Groothuis, GMM & Olinga, P 2020, 'Host microbiota dictates the proinflammatory impact of LPS in the murine liver', Toxicology in Vitro, bind 67, 104920. https://doi.org/10.1016/j.tiv.2020.104920

APA

Suriguga, S., Luangmonkong, T., Mutsaers, H. A. M., Groothuis, G. M. M., & Olinga, P. (2020). Host microbiota dictates the proinflammatory impact of LPS in the murine liver. Toxicology in Vitro, 67, [104920]. https://doi.org/10.1016/j.tiv.2020.104920

CBE

Suriguga S, Luangmonkong T, Mutsaers HAM, Groothuis GMM, Olinga P. 2020. Host microbiota dictates the proinflammatory impact of LPS in the murine liver. Toxicology in Vitro. 67:Article 104920. https://doi.org/10.1016/j.tiv.2020.104920

MLA

Vancouver

Suriguga S, Luangmonkong T, Mutsaers HAM, Groothuis GMM, Olinga P. Host microbiota dictates the proinflammatory impact of LPS in the murine liver. Toxicology in Vitro. 2020 sep;67. 104920. https://doi.org/10.1016/j.tiv.2020.104920

Author

Suriguga, Su ; Luangmonkong, Theerut ; Mutsaers, Henricus A.M. ; Groothuis, Geny M.M. ; Olinga, Peter. / Host microbiota dictates the proinflammatory impact of LPS in the murine liver. I: Toxicology in Vitro. 2020 ; Bind 67.

Bibtex

@article{072a84a51dc24520819efbba72f95780,
title = "Host microbiota dictates the proinflammatory impact of LPS in the murine liver",
abstract = "Gut microbiota can impact liver disease development via the gut-liver axis. Liver inflammation is a shared pathological event in various liver diseases and gut microbiota might influence this pathological process. In this study, we studied the influence of gut microbiota on the inflammatory response of the liver to lipopolysaccharide (LPS). The inflammatory response to LPS (1–10 μg/ml) of livers of specific-pathogen-free (SPF) or germ-free (GF) mice was evaluated ex vivo, using precision-cut liver slices (PCLS). LPS induced a more pronounced inflammatory response in GF PCLS than in SPF PCLS. Baseline TNF-α gene expression was significantly higher in GF slices as compared to SPF slices. LPS treatment induced TNF-α, IL-1β, IL-6 and iNOS expression in both SPF and GF PCLS, but the increase was more intense in GF slices. The anti-inflammatory markers SOCS3 and IRAK-M gene expression was significantly higher in GF PCLS than SPF PCLS at 24h with 1 µg/ml LPS treatment, and IL-10 was not differently expressed in GF PCLS than SPF PCLS. In addition, TLR-4 mRNA, but not protein, at basal level was higher in GF slices than in SPF slices. Taken together, this study shows that, in mice, the host microbiota attenuates the pro-inflammatory impact of LPS in the liver, indicating a positive role of the gut microbiota on the immune homeostasis of the liver.",
keywords = "Germ free mice, Liver, LPS, Microbiota, Precision-cut liver slices",
author = "Su Suriguga and Theerut Luangmonkong and Mutsaers, {Henricus A.M.} and Groothuis, {Geny M.M.} and Peter Olinga",
year = "2020",
month = sep,
doi = "10.1016/j.tiv.2020.104920",
language = "English",
volume = "67",
journal = "Toxicology in Vitro",
issn = "0887-2333",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Host microbiota dictates the proinflammatory impact of LPS in the murine liver

AU - Suriguga, Su

AU - Luangmonkong, Theerut

AU - Mutsaers, Henricus A.M.

AU - Groothuis, Geny M.M.

AU - Olinga, Peter

PY - 2020/9

Y1 - 2020/9

N2 - Gut microbiota can impact liver disease development via the gut-liver axis. Liver inflammation is a shared pathological event in various liver diseases and gut microbiota might influence this pathological process. In this study, we studied the influence of gut microbiota on the inflammatory response of the liver to lipopolysaccharide (LPS). The inflammatory response to LPS (1–10 μg/ml) of livers of specific-pathogen-free (SPF) or germ-free (GF) mice was evaluated ex vivo, using precision-cut liver slices (PCLS). LPS induced a more pronounced inflammatory response in GF PCLS than in SPF PCLS. Baseline TNF-α gene expression was significantly higher in GF slices as compared to SPF slices. LPS treatment induced TNF-α, IL-1β, IL-6 and iNOS expression in both SPF and GF PCLS, but the increase was more intense in GF slices. The anti-inflammatory markers SOCS3 and IRAK-M gene expression was significantly higher in GF PCLS than SPF PCLS at 24h with 1 µg/ml LPS treatment, and IL-10 was not differently expressed in GF PCLS than SPF PCLS. In addition, TLR-4 mRNA, but not protein, at basal level was higher in GF slices than in SPF slices. Taken together, this study shows that, in mice, the host microbiota attenuates the pro-inflammatory impact of LPS in the liver, indicating a positive role of the gut microbiota on the immune homeostasis of the liver.

AB - Gut microbiota can impact liver disease development via the gut-liver axis. Liver inflammation is a shared pathological event in various liver diseases and gut microbiota might influence this pathological process. In this study, we studied the influence of gut microbiota on the inflammatory response of the liver to lipopolysaccharide (LPS). The inflammatory response to LPS (1–10 μg/ml) of livers of specific-pathogen-free (SPF) or germ-free (GF) mice was evaluated ex vivo, using precision-cut liver slices (PCLS). LPS induced a more pronounced inflammatory response in GF PCLS than in SPF PCLS. Baseline TNF-α gene expression was significantly higher in GF slices as compared to SPF slices. LPS treatment induced TNF-α, IL-1β, IL-6 and iNOS expression in both SPF and GF PCLS, but the increase was more intense in GF slices. The anti-inflammatory markers SOCS3 and IRAK-M gene expression was significantly higher in GF PCLS than SPF PCLS at 24h with 1 µg/ml LPS treatment, and IL-10 was not differently expressed in GF PCLS than SPF PCLS. In addition, TLR-4 mRNA, but not protein, at basal level was higher in GF slices than in SPF slices. Taken together, this study shows that, in mice, the host microbiota attenuates the pro-inflammatory impact of LPS in the liver, indicating a positive role of the gut microbiota on the immune homeostasis of the liver.

KW - Germ free mice

KW - Liver

KW - LPS

KW - Microbiota

KW - Precision-cut liver slices

UR - http://www.scopus.com/inward/record.url?scp=85087031973&partnerID=8YFLogxK

U2 - 10.1016/j.tiv.2020.104920

DO - 10.1016/j.tiv.2020.104920

M3 - Journal article

C2 - 32590029

AN - SCOPUS:85087031973

VL - 67

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

M1 - 104920

ER -