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High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis

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High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis. / Yu, X.; Zhu, Z.; Zhang, T.; Li, J; Cheng, C.; Lou, Q.; Ottosen, Carl-Otto; Chen, J.

I: Biologia Plantarum, Bind 64, 2020, s. 104-109.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Yu, X, Zhu, Z, Zhang, T, Li, J, Cheng, C, Lou, Q, Ottosen, C-O & Chen, J 2020, 'High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis', Biologia Plantarum, bind 64, s. 104-109. https://doi.org/10.32615/bp.2019.121

APA

Yu, X., Zhu, Z., Zhang, T., Li, J., Cheng, C., Lou, Q., Ottosen, C-O., & Chen, J. (2020). High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis. Biologia Plantarum, 64, 104-109. https://doi.org/10.32615/bp.2019.121

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MLA

Vancouver

Author

Yu, X. ; Zhu, Z. ; Zhang, T. ; Li, J ; Cheng, C. ; Lou, Q. ; Ottosen, Carl-Otto ; Chen, J. / High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis. I: Biologia Plantarum. 2020 ; Bind 64. s. 104-109.

Bibtex

@article{7159efc70bf143ae87e54e743226debb,
title = "High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis",
abstract = "Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis xhytivus and its parents. The different developmental stages of leaves of C. xhytivus showing contrasting leaf colour were compared as well. Following high-throughput sequencing. 546 conserved d 287 novel miRNAs were identified. The expressions of nine miRNAs obtained by real-time quantitative PCR were consistent with the sequencing results. We detected that 15 miRNAs were divergently expressed between the parent species, and 23 miRNAs were differentially expressed in C. xbytivus compared to either of its parents or both, which suggests the significant effect of allopolyploidization on miRNAs accumulation. Additionally, 26 conserved and 13 novel miRNAs differed in expression between the young and mature leaves of C. xhytivus, indicating an essential role of miRNA-mediated regulation of leaf development following allopolyploidization. This study enriches the context of allopolyploidy effect on miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in phenotypic variation in allopolyploids.",
keywords = "COTTON, HYBRIDIZATION, INTERSPECIFIC HYBRIDS, POLYPLOIDY, SMALL RNA TRANSCRIPTOMES, cucumber, gene expression, leaf development, next-generation sequencing",
author = "X. Yu and Z. Zhu and T. Zhang and J Li and C. Cheng and Q. Lou and Carl-Otto Ottosen and J. Chen",
year = "2020",
doi = "10.32615/bp.2019.121",
language = "English",
volume = "64",
pages = "104--109",
journal = "Biologia Plantarum",
issn = "0006-3134",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis

AU - Yu, X.

AU - Zhu, Z.

AU - Zhang, T.

AU - Li, J

AU - Cheng, C.

AU - Lou, Q.

AU - Ottosen, Carl-Otto

AU - Chen, J.

PY - 2020

Y1 - 2020

N2 - Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis xhytivus and its parents. The different developmental stages of leaves of C. xhytivus showing contrasting leaf colour were compared as well. Following high-throughput sequencing. 546 conserved d 287 novel miRNAs were identified. The expressions of nine miRNAs obtained by real-time quantitative PCR were consistent with the sequencing results. We detected that 15 miRNAs were divergently expressed between the parent species, and 23 miRNAs were differentially expressed in C. xbytivus compared to either of its parents or both, which suggests the significant effect of allopolyploidization on miRNAs accumulation. Additionally, 26 conserved and 13 novel miRNAs differed in expression between the young and mature leaves of C. xhytivus, indicating an essential role of miRNA-mediated regulation of leaf development following allopolyploidization. This study enriches the context of allopolyploidy effect on miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in phenotypic variation in allopolyploids.

AB - Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis xhytivus and its parents. The different developmental stages of leaves of C. xhytivus showing contrasting leaf colour were compared as well. Following high-throughput sequencing. 546 conserved d 287 novel miRNAs were identified. The expressions of nine miRNAs obtained by real-time quantitative PCR were consistent with the sequencing results. We detected that 15 miRNAs were divergently expressed between the parent species, and 23 miRNAs were differentially expressed in C. xbytivus compared to either of its parents or both, which suggests the significant effect of allopolyploidization on miRNAs accumulation. Additionally, 26 conserved and 13 novel miRNAs differed in expression between the young and mature leaves of C. xhytivus, indicating an essential role of miRNA-mediated regulation of leaf development following allopolyploidization. This study enriches the context of allopolyploidy effect on miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in phenotypic variation in allopolyploids.

KW - COTTON

KW - HYBRIDIZATION

KW - INTERSPECIFIC HYBRIDS

KW - POLYPLOIDY

KW - SMALL RNA TRANSCRIPTOMES

KW - cucumber

KW - gene expression

KW - leaf development

KW - next-generation sequencing

U2 - 10.32615/bp.2019.121

DO - 10.32615/bp.2019.121

M3 - Journal article

VL - 64

SP - 104

EP - 109

JO - Biologia Plantarum

JF - Biologia Plantarum

SN - 0006-3134

ER -