High Throughput Identification and Quantification of Phospholipids in Complex Mixtures

Nicholas Mcleod Balsgart; Mette Mulbjerg; Zheng Guo; Kresten Bertelsen; Thomas Vosegaard

doi:10.1021/acs.analchem.5b03798

High Throughput Identification and Quantification of Phospholipids in Complex Mixtures

Nicholas Mcleod Balsgart, Mette Mulbjerg, Zheng Guo, Kresten Bertelsen, Thomas Vosegaard

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

28 Citationer (Scopus)

Abstract

We present a fully automatic method, autoP, for identification and quantification of lipids in complex lipid mixtures from 1D ³¹P and 2D ¹H- ³¹P NMR spectra. The ³¹P chemical shifts in lipids are highly sensitive to experimental conditions such as pH and temperature, so the present method uses the much more unambiguous ¹H chemical shifts for assignment and ³¹P intensities for quantification. By using 2D ¹H- ³¹P total correlation spectroscopy (TOCSY) correlation experiments, we demonstrate that approximately 20 different lipids can be automatically and unambiguously assigned and quantified by this automatic method.

Originalsprog	Engelsk
Tidsskrift	Analytical Chemistry
Vol/bind	88
Nummer	4
Sider (fra-til)	2170-2176
Antal sider	7
ISSN	0003-2700
DOI	https://doi.org/10.1021/acs.analchem.5b03798
Status	Udgivet - 16 feb. 2016

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title = "High Throughput Identification and Quantification of Phospholipids in Complex Mixtures",

abstract = "We present a fully automatic method, autoP, for identification and quantification of lipids in complex lipid mixtures from 1D 31P and 2D 1H- 31P NMR spectra. The 31P chemical shifts in lipids are highly sensitive to experimental conditions such as pH and temperature, so the present method uses the much more unambiguous 1H chemical shifts for assignment and 31P intensities for quantification. By using 2D 1H- 31P total correlation spectroscopy (TOCSY) correlation experiments, we demonstrate that approximately 20 different lipids can be automatically and unambiguously assigned and quantified by this automatic method.",

author = "Balsgart, {Nicholas Mcleod} and Mette Mulbjerg and Zheng Guo and Kresten Bertelsen and Thomas Vosegaard",

year = "2016",

month = feb,

day = "16",

doi = "10.1021/acs.analchem.5b03798",

language = "English",

volume = "88",

pages = "2170--2176",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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TY - JOUR

T1 - High Throughput Identification and Quantification of Phospholipids in Complex Mixtures

AU - Balsgart, Nicholas Mcleod

AU - Mulbjerg, Mette

AU - Guo, Zheng

AU - Bertelsen, Kresten

AU - Vosegaard, Thomas

PY - 2016/2/16

Y1 - 2016/2/16

N2 - We present a fully automatic method, autoP, for identification and quantification of lipids in complex lipid mixtures from 1D 31P and 2D 1H- 31P NMR spectra. The 31P chemical shifts in lipids are highly sensitive to experimental conditions such as pH and temperature, so the present method uses the much more unambiguous 1H chemical shifts for assignment and 31P intensities for quantification. By using 2D 1H- 31P total correlation spectroscopy (TOCSY) correlation experiments, we demonstrate that approximately 20 different lipids can be automatically and unambiguously assigned and quantified by this automatic method.

AB - We present a fully automatic method, autoP, for identification and quantification of lipids in complex lipid mixtures from 1D 31P and 2D 1H- 31P NMR spectra. The 31P chemical shifts in lipids are highly sensitive to experimental conditions such as pH and temperature, so the present method uses the much more unambiguous 1H chemical shifts for assignment and 31P intensities for quantification. By using 2D 1H- 31P total correlation spectroscopy (TOCSY) correlation experiments, we demonstrate that approximately 20 different lipids can be automatically and unambiguously assigned and quantified by this automatic method.

UR - http://www.scopus.com/inward/record.url?scp=84958259663&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.5b03798

DO - 10.1021/acs.analchem.5b03798

M3 - Journal article

C2 - 26797086

SN - 0003-2700

VL - 88

SP - 2170

EP - 2176

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 4

ER -

High Throughput Identification and Quantification of Phospholipids in Complex Mixtures

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