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High blood oxygen affinity in the air-breathing swamp eel Monopterus albus

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High blood oxygen affinity in the air-breathing swamp eel Monopterus albus. / Damsgaard, Christian; Findorf, Inge; Helbo, Signe et al.
I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Bind 178, 12.2014, s. 102-108.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Damsgaard, C, Findorf, I, Helbo, S, Kocagoz, Y, Buchanan, R, Huong, DTT, Weber, RE, Fago, A, Bayley, M & Wang, T 2014, 'High blood oxygen affinity in the air-breathing swamp eel Monopterus albus', Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, bind 178, s. 102-108. https://doi.org/10.1016/j.cbpa.2014.08.001

APA

Damsgaard, C., Findorf, I., Helbo, S., Kocagoz, Y., Buchanan, R., Huong, D. T. T., Weber, R. E., Fago, A., Bayley, M., & Wang, T. (2014). High blood oxygen affinity in the air-breathing swamp eel Monopterus albus. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 178, 102-108. https://doi.org/10.1016/j.cbpa.2014.08.001

CBE

Damsgaard C, Findorf I, Helbo S, Kocagoz Y, Buchanan R, Huong DTT, Weber RE, Fago A, Bayley M, Wang T. 2014. High blood oxygen affinity in the air-breathing swamp eel Monopterus albus. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 178:102-108. https://doi.org/10.1016/j.cbpa.2014.08.001

MLA

Damsgaard, Christian et al. "High blood oxygen affinity in the air-breathing swamp eel Monopterus albus". Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 2014, 178. 102-108. https://doi.org/10.1016/j.cbpa.2014.08.001

Vancouver

Damsgaard C, Findorf I, Helbo S, Kocagoz Y, Buchanan R, Huong DTT et al. High blood oxygen affinity in the air-breathing swamp eel Monopterus albus. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 2014 dec.;178:102-108. doi: 10.1016/j.cbpa.2014.08.001

Author

Damsgaard, Christian ; Findorf, Inge ; Helbo, Signe et al. / High blood oxygen affinity in the air-breathing swamp eel Monopterus albus. I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 2014 ; Bind 178. s. 102-108.

Bibtex

@article{bc2546ebe10941f494bf80d5da41da87,
title = "High blood oxygen affinity in the air-breathing swamp eel Monopterus albus",
abstract = "The Asian swamp eel (Monopterus albus, Zuiew 1793) is a facultative air-breathing fish with reduced gills. Previous studies have shown that gas exchange seems to occur across the epithelium of the buccopharyngeal cavity, the esophagus and the integument, resulting in substantial diffusion limitations that must be compensated by adaptations in others steps of the O₂ transport system to secure adequate O₂ delivery to the respiring tissues. We therefore investigated O₂ binding properties of whole blood, stripped hemoglobin (Hb), two major isoHb components and the myoglobin (Mb) from M. albus. Whole blood was sampled using indwelling catheters for blood gas analysis and determination of O₂ equilibrium curves. Hb was purified to assess the effects of endogenous allosteric effectors, and Mb was isolated from heart and skeletal muscle to determine its O₂ binding properties. The blood of M. albus has a high O₂ carrying capacity [hematocrit (Hct) of 42.4±4.5%] and binds O₂ with an unusually high affinity (P₅₀=2.8±0.4mmHg at 27°C and pH7.7), correlating with insensitivity of the Hb to the anionic allosteric effectors that normally decrease Hb-O₂ affinity. In addition, Mb is present at high concentrations in both heart and muscle (5.16±0.99 and 1.08±0.19mg ∙ g wet tissue⁻¹, respectively). We suggest that the high Hct and high blood O₂ affinity serve to overcome the low diffusion capacity in the relatively inefficient respiratory surfaces, while high Hct and Mb concentration aid in increasing the O₂ flux from the blood to the muscles.",
keywords = "Algorithms, Allosteric Regulation, Animals, Aquaculture, Biological Transport, Fish Proteins, Hematocrit, Hemoglobins, Hydrogen-Ion Concentration, Kinetics, Models, Biological, Muscle, Skeletal, Myocardium, Myoglobin, Oxygen, Oxygen Consumption, Protein Isoforms, Respiratory Mucosa, Smegmamorpha, Vietnam, Journal Article, Research Support, Non-U.S. Gov't",
author = "Christian Damsgaard and Inge Findorf and Signe Helbo and Yigit Kocagoz and Rasmus Buchanan and Huong, {Do Thi Thanh} and Weber, {Roy E.} and Angela Fago and Mark Bayley and Tobias Wang",
note = "Copyright {\textcopyright} 2014 Elsevier Inc. All rights reserved.",
year = "2014",
month = dec,
doi = "10.1016/j.cbpa.2014.08.001",
language = "English",
volume = "178",
pages = "102--108",
journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
issn = "1095-6433",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - High blood oxygen affinity in the air-breathing swamp eel Monopterus albus

AU - Damsgaard, Christian

AU - Findorf, Inge

AU - Helbo, Signe

AU - Kocagoz, Yigit

AU - Buchanan, Rasmus

AU - Huong, Do Thi Thanh

AU - Weber, Roy E.

AU - Fago, Angela

AU - Bayley, Mark

AU - Wang, Tobias

N1 - Copyright © 2014 Elsevier Inc. All rights reserved.

PY - 2014/12

Y1 - 2014/12

N2 - The Asian swamp eel (Monopterus albus, Zuiew 1793) is a facultative air-breathing fish with reduced gills. Previous studies have shown that gas exchange seems to occur across the epithelium of the buccopharyngeal cavity, the esophagus and the integument, resulting in substantial diffusion limitations that must be compensated by adaptations in others steps of the O₂ transport system to secure adequate O₂ delivery to the respiring tissues. We therefore investigated O₂ binding properties of whole blood, stripped hemoglobin (Hb), two major isoHb components and the myoglobin (Mb) from M. albus. Whole blood was sampled using indwelling catheters for blood gas analysis and determination of O₂ equilibrium curves. Hb was purified to assess the effects of endogenous allosteric effectors, and Mb was isolated from heart and skeletal muscle to determine its O₂ binding properties. The blood of M. albus has a high O₂ carrying capacity [hematocrit (Hct) of 42.4±4.5%] and binds O₂ with an unusually high affinity (P₅₀=2.8±0.4mmHg at 27°C and pH7.7), correlating with insensitivity of the Hb to the anionic allosteric effectors that normally decrease Hb-O₂ affinity. In addition, Mb is present at high concentrations in both heart and muscle (5.16±0.99 and 1.08±0.19mg ∙ g wet tissue⁻¹, respectively). We suggest that the high Hct and high blood O₂ affinity serve to overcome the low diffusion capacity in the relatively inefficient respiratory surfaces, while high Hct and Mb concentration aid in increasing the O₂ flux from the blood to the muscles.

AB - The Asian swamp eel (Monopterus albus, Zuiew 1793) is a facultative air-breathing fish with reduced gills. Previous studies have shown that gas exchange seems to occur across the epithelium of the buccopharyngeal cavity, the esophagus and the integument, resulting in substantial diffusion limitations that must be compensated by adaptations in others steps of the O₂ transport system to secure adequate O₂ delivery to the respiring tissues. We therefore investigated O₂ binding properties of whole blood, stripped hemoglobin (Hb), two major isoHb components and the myoglobin (Mb) from M. albus. Whole blood was sampled using indwelling catheters for blood gas analysis and determination of O₂ equilibrium curves. Hb was purified to assess the effects of endogenous allosteric effectors, and Mb was isolated from heart and skeletal muscle to determine its O₂ binding properties. The blood of M. albus has a high O₂ carrying capacity [hematocrit (Hct) of 42.4±4.5%] and binds O₂ with an unusually high affinity (P₅₀=2.8±0.4mmHg at 27°C and pH7.7), correlating with insensitivity of the Hb to the anionic allosteric effectors that normally decrease Hb-O₂ affinity. In addition, Mb is present at high concentrations in both heart and muscle (5.16±0.99 and 1.08±0.19mg ∙ g wet tissue⁻¹, respectively). We suggest that the high Hct and high blood O₂ affinity serve to overcome the low diffusion capacity in the relatively inefficient respiratory surfaces, while high Hct and Mb concentration aid in increasing the O₂ flux from the blood to the muscles.

KW - Algorithms

KW - Allosteric Regulation

KW - Animals

KW - Aquaculture

KW - Biological Transport

KW - Fish Proteins

KW - Hematocrit

KW - Hemoglobins

KW - Hydrogen-Ion Concentration

KW - Kinetics

KW - Models, Biological

KW - Muscle, Skeletal

KW - Myocardium

KW - Myoglobin

KW - Oxygen

KW - Oxygen Consumption

KW - Protein Isoforms

KW - Respiratory Mucosa

KW - Smegmamorpha

KW - Vietnam

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.cbpa.2014.08.001

DO - 10.1016/j.cbpa.2014.08.001

M3 - Journal article

C2 - 25139401

VL - 178

SP - 102

EP - 108

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

SN - 1095-6433

ER -