Hairy roots - a short cut to transgenic root nodules

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DOI

  • Jørgen Hansen, Department of Molecular Biology and Plant Physiology
  • ,
  • Jan Elo Jørgensen, Department of Molecular Biology and Plant Physiology
  • ,
  • Jens Stougaard
  • Kjeld A. Marcker, Department of Molecular Biology and Plant Physiology

To facilitate molecular studies of symbiotic nitrogen fixation a procedure for rapid production of transgenic root nodules was established on the legume Lotus corniculatus (Bird'sfoot trefoil). Regeneration of transgenic plants is not required as transgenic nodules are formed on Agrobacterium rhizogenes incited roots inoculated with Rhizobium. Easy identification of transformed roots is possible using a set of A. rhizogenes acceptor strains carrying assayable marker genes such as chloramphenicol acetyltransferase (CAT), β-glucuronidase (GUS), or luciferase (LUC) under control of the cauliflower mosaic virus (CaMV) 35S promoter. Counterselection of A. rhizogenes after infection of plants was improved using an auxotrophy marker.

OriginalsprogEngelsk
TidsskriftPlant Cell Reports
Vol/bind8
Nummer1
Sider (fra-til)12-15
Antal sider4
ISSN0721-7714
DOI
StatusUdgivet - maj 1989

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