Haemoglobin modulates salicylate and jasmonate/ethylene-mediated resistance mechanisms against pathogens

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  • Luis A J Mur, Aberystwyth University, Institute of Biological, Environmental and Rural Sciences, Storbritannien
  • Anushen Sivakumaran, Aberystwyth University, Institute of Biological, Environmental and Rural Sciences, Storbritannien
  • Julien Mandon, Department of Molecular and Laser Physics, Radboud University Nijmegen, Holland
  • Simona M Cristescu, Department of Molecular and Laser Physics, Radboud University Nijmegen, Holland
  • Frans J M Harren, Department of Molecular and Laser Physics, Radboud University Nijmegen, Holland
  • Kim Hebelstrup

Nitric oxide (NO) plays a role in defence against hemibiotrophic pathogens mediated by salicylate (SA) and also necrotrophic pathogens influenced by jasmonate/ethylene (JA/Et). This study examined how NO-oxidizing haemoglobins (Hb) encoded by GLB1, GLB2, and GLB3 in Arabidopsis could influence both defence pathways. The impact of Hb on responses to the hemibiotrophic Pseudomonas syringae pathovar tomato (Pst) AvrRpm1 and the necrotrophic Botrytis cinerea were investigated using glb1, glb2, and glb3 mutant lines and also CaMV 35S GLB1 and GLB2 overexpression lines. In glb1, but not glb2 and glb3, increased resistance was observed to both pathogens but was compromised in the 35S-GLB1. A quantum cascade laser-based sensor measured elevated NO production in glb1 infected with Pst AvrRpm1 and B. cinerea, which was reduced in 35S-GLB1 compared to Col-0. SA accumulation was increased in glb1 and reduced in 35S-GLB1 compared to controls following attack by Pst AvrRpm1. Similarly, JA and Et levels were increased in glb1 but decreased in 35S-GLB1 in response to attack by B. cinerea. Quantitative PCR assays indicated reduced GLB1 expression during challenge with either pathogen, thus this may elevate NO concentration and promote a wide-ranging defence against pathogens.

OriginalsprogEngelsk
TidsskriftJournal of Experimental Botany
Vol/bind63
Nummer12
Sider (fra-til)4375-4387
Antal sider13
ISSN0022-0957
DOI
StatusUdgivet - jul. 2012

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